FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58340.373030305355421No Hit
GTGTGTATGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC54920.3511625706225527No Hit
ACATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT44290.28319355886512854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42100.2691905357467128No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39620.2533332310281416No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38350.24521275643435717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37990.242910889620371No Hit
CCCTTGGACAGCCGGCCTCCGTCTCCTGCAGGTCTAGCCAAGGCCTCGAA36900.2359413484335796No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34430.22014798445984138No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33820.21624759902503154No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG28230.18050472266341336No Hit
CATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27390.1751337000974457No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27360.17494187786294685No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27320.17468611488361507No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27180.17379094445595378No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26830.1715530183868006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26160.16726898848299304No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC26020.16637381805533177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25740.1645834772000092No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25320.16189796591702538No Hit
GTGTATGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC25310.16183402517219245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25210.16119461772386293No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24810.158636987930545No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGTTGGACATATCCAAATACA24790.15850910644087912No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24580.15716635079938718No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA24550.15697452856488833No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24540.1569105878200554No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC22490.14380273512930097No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22330.1427796832119738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21800.13939082373582753No Hit
CAACATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21700.13875141628749804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21690.1386874755426651No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21530.13766442362533793No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21010.1343395048940246No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20560.13146217137654193No Hit
GGCCAGGCCAATCTCCCAGGCGCCTAATTTATAAGGTTTCTAATCGCGAC20500.13107852690754423No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCGTCTCCTGCAGGTCTAGCCAAGGCCTCGAAGA20050.12820119339006156No Hit
GTATATTACTGTCTGGCCTGGGGGGTGAAAAACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCC19830.1267944970037367No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19810.1266666155140708No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT19430.12423686721041877No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19260.12314987454825864No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18860.1205922447549407No Hit
GGTCTAGCCAAGGCCTCGAAGACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC18800.12020860028594302No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18700.11956919283761354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18680.11944131134794764No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA18310.11707550378912855No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18310.11707550378912855No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAACATACACACTGGCCTCACACTTTTGGCCAGGGGA18290.11694762229946265No Hit
GGCCAAAAGTGTGAGGCCAGTGTGTATGTTGCATGCAGTAATAAACCCCA18070.11554092591313779No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC17740.11343088133365048No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCGTCTCCTGCAGGTCTA17470.11170448122316087No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17020.1088271477056782No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCCAGGCGCCTAATTTATAAGGTTT17010.10876320696084525No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16830.10761227355385217No Hit
GTTTATTACTGCATGCAACATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15700.100386969387729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA100.007498036142.3146570
GCTACGC252.7635475E-454.38363320
GTCGATA200.00783275950.94062813
GTCGATT356.843315E-445.25136668
TGGTATC5350.045.1050342
GTGGTAT5500.044.511381
GGTATCA15200.040.041531
ATACCGT558.255194E-637.0382546
GTATCAA34900.036.8271031
CTTATCG400.00240586235.04087451
AATGGTA5600.034.30870
TATCAAC40650.031.5210912
TCTGGAG15900.030.87959770
ATCAACG41800.030.7967873
AACGCAG42550.030.400966
TCAACGC42500.030.122964
CAACGCA42800.030.0656955
ACGCAGA44350.029.16717
CGCAGAG44900.028.6585888
GACAGGT3250.028.2476021
Produced by FastQC (version 0.11.5)