FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859818
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT59780.32142930114667134No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATT59450.3196549339774107No Hit
GATCTATGGTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA56500.30379316685826246No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55930.3007283508386304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53060.2852967333362727No Hit
GTTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA48760.26217619143378545No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47500.2554013349693357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT47070.253089280779087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42540.22873205872832716No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40820.21948384196733228No Hit
CTATAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC40050.2153436519057241No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39730.21362305343856228No Hit
GTACTATAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT38590.20749342139929822No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCAGTCATTTAAATTGGTATCAACAAAAA35500.1908788924507667No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33640.18087791386038848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33520.1802326894352028No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG32680.17571611845890298No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTT32170.1729739146518638No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31620.17001663603642936No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31240.16797342535667467No Hit
CCTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAATGACTGCTA30840.16582267727272237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30470.16383323529506652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29470.15845636508518576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28820.15496139944876328No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28680.15420863761937997No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC28530.15340210708789784No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28470.153079494875305No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28410.15275688266271215No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27430.14748754985702903No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATA27300.14678855672974453No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26540.14270213537023516No Hit
GTCCTGATCTATGGTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT25940.1394760132443067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25800.1387232514149234No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAAT25570.13748657126665081No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24800.13334638120504264No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24130.12974387816442254No Hit
GTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA23720.12753936137837144No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA23590.12684036825108694No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23000.12366801482725728No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTATAGTACC22840.12280771559367637No Hit
GGGTACTATAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT22670.12189364765799664No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22550.12124842323281095No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21930.11791476370268487No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21660.11646300874601709No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT21630.11630170263972066No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT21620.11624793393762184No Hit
ATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA21530.11576401561873258No Hit
ACTTACTACTGTCAACAGAGTTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21420.1151725598956457No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21230.11415095455576836No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21100.11345196142848385No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20930.11253789349280413No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGA20890.1123228186844089No Hit
GTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATACA20590.11070975762144468No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20120.10818262862280072No Hit
GTATTACTGTGCGAGAGTGGAGGACCAAACCGTACGAAACTCCTACTATT19600.10538665611366273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19080.10259068360452474No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18800.10108515994575813No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18800.10108515994575813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT950.046.9341339
CGTATGC12800.044.76161241
TCGTATG12000.044.733640
GTCGTAT2650.044.00501339
TCGACGT19100.043.48711429
GTATGCC13650.043.37391742
TATACAC19700.043.2862853
CTTATAC19400.043.2723431
TTATACA19450.043.157622
ACTCGAC19150.043.1065927
CTCGACG19450.042.8327528
ACGAGAC19450.042.8289222
AGACTCG19300.042.69093325
CGACGTC19450.042.60610630
GACTCGA19350.042.4493926
ATGCCGT13050.042.41559244
CCGTCTT12200.042.39820547
TCGCGTA1050.042.3095738
CACGAGA19700.042.11421
ACGTCAT17200.042.07102232
Produced by FastQC (version 0.11.5)