FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859818
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180390.9699336171603888No Hit
GAGTTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT57740.3104604859185146No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53930.2899746104188689No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATT52670.2831997539544192No Hit
GATCTATGGTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA51770.2783605707655265No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG46940.2523902876518025No Hit
GTTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA43660.23475415336339364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41550.2234089572205452No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39770.2138381282469575No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39640.213139135119673No Hit
GTACTATAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT38080.20475121759225903No Hit
CTATAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC37140.19969695959497114No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37050.19921304127608186No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCAGTCATTTAAATTGGTATCAACAAAAA33410.1796412337121159No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32440.1744256696085316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30680.16496237803914146No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30600.164532228422351No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29190.15695084142641913No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTT28190.15157397121653837No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28170.15146643381234076No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28140.15130512770604435No Hit
CCTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAATGACTGCTA27940.1502297536640682No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC27770.14931568572838846No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27720.14904684221789444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27190.14619710100665764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26980.14506795826258267No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26970.1450141895604839No Hit
GTCCTGATCTATGGTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT26470.14232575445554352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26160.14065892469048047No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26080.14022877507369No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25280.1359272789057854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23650.12716298046367977No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA23390.12576499420911078No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAAT23370.12565745680491316No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22940.12334540261466445No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22730.1222162598705895No Hit
GGGTACTATAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT22680.12194741636009546No Hit
GTAGTAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22630.12167857284960142No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22460.12076450491392168No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22300.11990420568034077No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATA22220.1194740560635503No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22160.11915144385095747No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTACTACTGTCAACAGAGTTATAGTACC21680.1165705461502147No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21460.11538763470404093No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGA20950.11264543089700176No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT20260.10893539045218403No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19960.1073223293892198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19920.10710725458082458No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19320.10388113245489612No Hit
ATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA19280.1036660576465009No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19260.10355852024230329No Hit
ACTTACTACTGTCAACAGAGTTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19240.10345098283810565No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18940.10183792177514144No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18670.10038616681847362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.049.2124821
ATCAACG43050.031.3003333
AACGCAG43350.031.2195826
TCAACGC43400.031.1852914
CAACGCA43800.030.8229145
ACGCAGA45350.029.98947
CGCAGAG45600.029.8249848
AGAGTAC46000.029.34798411
GCAGAGT47650.028.4022859
GTCGTAA855.503556E-627.63017330
GCTTGAT2500.025.3010081
TGTTAGA21200.025.02746470
AATGGTA5550.024.88229870
GAGTACT9700.024.34062612
TTCTAGT1500.00147237524.22749970
TACGGGC5750.023.15009115
GAGTACG48450.022.99302712
AGTACGG48650.022.83813313
AGTACTT9950.022.73436413
TCTCGCG9450.022.30468270
Produced by FastQC (version 0.11.5)