FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319893
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATGGTGCTCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT87910.6660388379967164No Hit
CCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGCGAGAGTGAAATCTGTC85680.6491435290587949No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64820.49110041495787915No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGCATCCGGGGTCCCT61820.4683712997947561No Hit
GCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAACCTAAGAAATTCAGATTTTTGGCGGTGGACAAAACACT58690.4446572563078977No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53280.40366908529706574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49800.377303311707843No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACTCTCGCCATCAGCAGCC47680.361241403659236No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGCGAGAGTGAAATCTGTCCCTGACCCGCTGCCA42220.319874414062352No Hit
GTCCCTGACCGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACTCTCGC42100.3189652494558271No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41460.31411637155436084No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40880.3097220759561571No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38290.2900992731986608No Hit
GTTTATTACTGTCAACAATATTATGGTGCTCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA35540.269264250965798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34970.26494571908480463No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGC33200.25153554113856197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33110.2508536676836683No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32080.24305000481099603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32050.2428227136593648No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30420.23047322775406795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30400.2303217003196471No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30360.2300186454508055No Hit
GTAATATACAGCCGTATCGTCGACTCTCAGGCTGCTCATCTGCAGATACA29480.22335143833628937No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29460.22319991090186858No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29380.22259380116418526No Hit
AGTCTAGCCAGAGTGTTTTGTCCACCGCCAAAAATCTGAATTTCTTAGGTTGGTACCAGCAGAAAGCAGGACAGC28590.21660846750456286No Hit
GTTTTGTCCACCGCCAAAAATCTGAATTTCTTAGGTTGGTACCAGCAGAA28290.21433555598825058No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28010.2122141719063591No Hit
CCACTGAACCGGTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGGAAAT27480.20819869489420734No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG26930.20403169044763478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26580.20137996034527042No Hit
CCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG26550.2011526691936392No Hit
GTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTC26370.1997889222838518No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTA25960.19668260987822497No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC25480.1930459514521253No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24870.18842436470229026No Hit
AAGCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGCA24110.18266632219429907No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTCCACCGCCAAAAATCTGAATTT23890.18099952041567008No Hit
TCTTCAGCCTGCAGGCTGCTGATGGCGAGAGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGGTCAGGGACC23550.17842355403051613No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22990.17418078586673313No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22330.16918038053084605No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC21850.16554372210474635No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21840.16546795838753595No Hit
GGTGATGGGAGCACCATAATATTGTTGACAGTAATAAACTGCCACATCTT21790.1650891398014839No Hit
GATTTCACTCTCGCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTGCT21790.1650891398014839No Hit
CTACGGGGACTTCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG21700.16440726634659023No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21360.16183129996143628No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21330.16160400880980502No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20890.158270405252547No Hit
GTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGTA20720.15698242205997No Hit
GTCCTGCTTTCTGCTGGTACCAACCTAAGAAATTCAGATTTTTGGCGGTG20580.1559217300190243No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20540.15561867515018263No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20470.15508832912970977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20300.1538003459371328No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20230.1532699999166599No Hit
GTCGTGTCCCTTGGCCGAAGGTGATGGGAGCACCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC19820.1501636875110331No Hit
GTCCCTGACCCGCTGCCACTGAACCGGTCAGGGACCCCGGATGCCCGGGT19680.14910299547008735No Hit
GGATCTCTGGTGCCTACGGGGACTTCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19550.148118067146352No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19150.1450875184579356No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18880.14304189809325452No Hit
GCTCATTTCCTGGGCATCTACCCGGGCATCCGGGGTCCCTGACCGGTTCAGTGGCAGCGGGTCAGGGACAGATTT17910.13569281752384474No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17770.134632125482899No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17770.134632125482899No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17340.13137428564285136No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17130.12978324758143275No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16780.12713151747906837No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTGCTCCCATCACCTTCGGCCAAGGGACACGA16680.12637388030696428No Hit
GTATATTACTGTGCGAGAGGAGACGGCCCGGGCAGTTATCTGATTGACTA16630.12599506172091224No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16520.12516166083159772No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16440.12455555109391443No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16410.12432825994228319No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16180.12258569444644377No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16030.12144923868828762No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16000.12122194753665638No Hit
GTGTTTTGTCCACCGCCAAAAATCTGAATTTCTTAGGTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTG15950.12084312895060433No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15900.12046431036455227No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15820.11985820062686899No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15790.11963090947523776No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15560.11788834397939833No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15470.11720647052450464No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15340.1162215422007693No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15260.11561543246308603No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15240.1154639050286652No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14930.11311522979514249No Hit
GGTCAGGGACAGATTTCACTCTCGCCATCAGCAGCCTGCAGGCTGAAGAT14880.11273641120909043No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14840.1124333563402488No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14830.11235759262303838No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14730.11159995545093429No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14590.11053926340998854No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14510.10993315367230526No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14260.108039060742045No Hit
GTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGCATCCGGGGTCCCTGA14220.10773600587320337No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACTTCGTGATGA14150.10720565985273048No Hit
ACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTCCACCGCCAAAAATCT14100.10682684126667842No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14040.10637225896341597No Hit
GCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGCATCCGGGGTCCCTGACCGGTT13930.10553885807410145No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13900.10531156692247022No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13850.10493274833641819No Hit
GCCACTGAACCGGTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTG13760.10425087488152447No Hit
GGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCT13410.10159914477916013No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13250.10038692530379356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)