FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319893
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATGGTGCTCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT81100.614443746576427No Hit
CCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGCGAGAGTGAAATCTGTC76250.577698343729378No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62580.4741293423027473No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGCATCCGGGGTCCCT56670.42935298543139483No Hit
GCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAACCTAAGAAATTCAGATTTTTGGCGGTGGACAAAACACT50290.38101573385115306No Hit
GTTCAGTGGCAGCGGGTCAGGGACAGATTTCACTCTCGCCATCAGCAGCC42110.3190410131730375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41930.31767726626325016No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41610.315252827312517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41330.31313144323062553No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGCGAGAGTGAAATCTGTCCCTGACCCGCTGCCA40640.30790374674310717No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40520.30699458213658226No Hit
GTCCCTGACCGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACTCTCGC36250.2746434748877371No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36000.27274938195747683No Hit
GTTTATTACTGTCAACAATATTATGGTGCTCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA34510.2614605880931258No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30650.23221579324990738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28410.2152447205947755No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28260.21410826483661935No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGC28050.2125172267752007No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27860.2110777161482029No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27620.20925938693515306No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27560.2088048046318906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26780.2028952346894786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26320.19941010369779974No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26190.1984251753740644No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG25740.19501580809959596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25740.19501580809959596No Hit
GTTTTGTCCACCGCCAAAAATCTGAATTTCTTAGGTTGGTACCAGCAGAA25640.19425817092749184No Hit
GTAATATACAGCCGTATCGTCGACTCTCAGGCTGCTCATCTGCAGATACA25300.1916822045423379No Hit
GTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTC25150.19054574878418173No Hit
AGTCTAGCCAGAGTGTTTTGTCCACCGCCAAAAATCTGAATTTCTTAGGTTGGTACCAGCAGAAAGCAGGACAGC25140.19046998506697135No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC24940.18895471072276315No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24720.1872879089441341No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24690.18706061779250288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24130.18281784962871991No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTA24090.18251479475987828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23730.17978730094030348No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23000.17425654958394354No Hit
CCACTGAACCGGTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGGAAAT22410.16978649026852935No Hit
GGTGATGGGAGCACCATAATATTGTTGACAGTAATAAACTGCCACATCTT21400.1621343548302779No Hit
CCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21350.16175553624422587No Hit
TCTTCAGCCTGCAGGCTGCTGATGGCGAGAGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGGTCAGGGACC21320.16152824509259464No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCTAGCCAGAGTGTTTTGTCCACCGCCAAAAATCTGAATTT21180.1604675530516489No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21080.15970991587954478No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21060.15955838844512396No Hit
GTCCCTGACCCGCTGCCACTGAACCGGTCAGGGACCCCGGATGCCCGGGT21020.15925533357628233No Hit
GATTTCACTCTCGCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTGCT20980.1589522787074407No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20480.15516409284692018No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20460.15501256541249933No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19740.14955757777334983No Hit
CTACGGGGACTTCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19700.14925452290450816No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19630.1487241768840353No Hit
AAGCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGCA19500.14773924856029996No Hit
GTCGTGTCCCTTGGCCGAAGGTGATGGGAGCACCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC19470.14751195740866874No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19310.14629973793330217No Hit
GGATCTCTGGTGCCTACGGGGACTTCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19280.14607244678167094No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18900.14319342552767533No Hit
GTCCTGCTTTCTGCTGGTACCAACCTAAGAAATTCAGATTTTTGGCGGTG18820.14258731578999206No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18790.14236002463836084No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTGCTCCCATCACCTTCGGCCAAGGGACACGA18620.14107204144578386No Hit
GCTCATTTCCTGGGCATCTACCCGGGCATCCGGGGTCCCTGACCGGTTCAGTGGCAGCGGGTCAGGGACAGATTT18350.1390264210811028No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18150.1375111467368946No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17670.1338744883107949No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17470.1323592139665867No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17040.12910137412653905No Hit
GTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGTA16710.1266011714585955No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16600.12576777056928098No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15880.12031278293013145No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15820.11985820062686899No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15820.11985820062686899No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15720.1191005634547649No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15650.11857021743429201No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15520.1175852891105567No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15480.11728223424171504No Hit
GAGTGAAATCTGTCCCTGACCCGCTGCCACTGAACCGGTCAGGGACCCCG15340.1162215422007693No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15270.11569119618029644No Hit
GTGTTTTGTCCACCGCCAAAAATCTGAATTTCTTAGGTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTG15150.11478203157377152No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15020.11379710325003618No Hit
GTATATTACTGTGCGAGAGGAGACGGCCCGGGCAGTTATCTGATTGACTA14900.11288793864351127No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACTTCGTGATGA14770.11190301031977591No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14610.11069079084440937No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14280.10819058817646582No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14240.10788753330762417No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14140.10712989613552008No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14060.10652378639783679No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13980.1059176766601535No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13960.1057661492257327No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13680.10364476514384122No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13590.1029628916889475No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG13340.10106879875868727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTA250.006439433453.5262269
GTGGTAT3150.045.5682871
TCTGGAG9350.043.67700670
GGTATCA9850.041.9829671
GTATCAA27200.041.2124941
TGGTATC3500.040.0228542
TAGTACG350.001417631339.0259744
TATCGTC7000.035.63010415
CGACTCT6800.035.18540221
TATCAAC32250.034.7485282
TCGACTC6950.034.424720
CGTCGAC7050.034.41599318
TTAACGG601.3853107E-534.3429335
ATCAACG32950.034.2021333
ATCGTCG7300.034.1684416
GTATCGT7100.034.15937414
TCAACGC32500.034.042664
TCGTCGA7250.033.93665717
CGTATCG7200.033.68366213
GTCGACT7150.033.45923619
Produced by FastQC (version 0.11.5)