FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082469
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGCTCACCCTTTATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA65180.602141955104488No Hit
CAGTAATACACTGCAAAATCTTCAGGGTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA47640.44010498222119987No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44950.415254386037845No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATACCA39460.3645369982881727No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36670.33876258812030646No Hit
GTGTATTACTGTCAGCAATATGGTAGCTCACCCTTTATCACTTTCGGCCC30970.2861051910031604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28830.2663355717346178No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27620.25515742252203066No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGTTACTTAGCCTGGTACC26930.24878310602890244No Hit
GTAATACACTGCAAAATCTTCAGGGTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26410.2439792733094435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23640.21838962593847952No Hit
CATTTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23170.21404770021127625No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23160.21395531881282515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23110.21349341182056947No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22470.2075810023196969No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT22220.20527146735841856No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG22020.2034238393893959No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21580.19935905785754604No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGACCCTGAAGATT21330.1970495228962677No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACTGCTGGTAACACTCTGA21310.1968647600993654No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA21310.1968647600993654No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC21270.1964952345055609No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20620.1904904436062372No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20430.18873519703566569No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20350.1879961458480566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20120.18587137368368054No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATAC19460.17977420138590572No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19360.1788503874013944No Hit
CCCTTTATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC18690.17266083370516846No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC18420.17016653594698786No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATAAAGGGTGAGCTACCATATTG17930.16563984742288232No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17820.16462365203991985No Hit
GTCAGAGTGTTACCAGCAGTTACTTAGCCTGGTACCAGCAGAAACCTGGC17570.16231411707864152No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA17190.15880362393749844No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16650.15381502842113723No Hit
GACTTCACTCTCACCATCACCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGCTCA16600.15335312142888158No Hit
CCCCAGCGCAGCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15670.14476165137292615No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15460.14282164200545233No Hit
GAGTGTTACCAGCAGTTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG14940.13801780928599341No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14840.13709399530148209No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14800.13672446970767754No Hit
TCACCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATAT14540.13432255334794807No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.13312159516808333No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14300.13210539978512087No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14190.1310892044021584No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14170.13090444160525613No Hit
GTGATAAAGGGTGAGCTACCATATTGCTGACAGTAATACACTGCAAAATC13450.12425298091677452No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12600.11640056204842819No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12500.11547674806391683No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12390.11446055268095438No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12270.11335197589954077No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12210.11279768750883395No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTT12170.11242816191502944No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11830.1092871943676909No Hit
ACCATATTGCTGACAGTAATACACTGCAAAATCTTCAGGGTCCAGTCTGG11750.10854814318008182No Hit
CTCCCAGGCTCCTCATTTATGGTGCATCCAACAGGGCCACTGGCATCCCA11600.10716242220331483No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11600.10716242220331483No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC11490.10614622682035237No Hit
CACCATCACCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGCTCACCCTTTATCA11320.10457574304668309No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11130.10282049647611156No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11110.10263573367920928No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAACTGCTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC11060.10217382668695361No Hit
GATAAAGGGTGAGCTACCATATTGCTGACAGTAATACACTGCAAAATCTT11030.10189668249160024No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCAACAGGGCCACTGGCATC10990.10152715689779569No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGGTGCATCCAACAGGGCCA10960.10125001270244229No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10940.10106524990554003No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10920.10088048710863776No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10910.10078810571018662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)