FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082469
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGCTCACCCTTTATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA58330.5388606971654616No Hit
CAGTAATACACTGCAAAATCTTCAGGGTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA43160.3987181157150921No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43020.39742477613677624No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATACCA34710.32065583402388426No Hit
GTGTATTACTGTCAGCAATATGGTAGCTCACCCTTTATCACTTTCGGCCC28370.2620860274058656No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27090.2502612084041206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26110.2412078313559095No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24970.23067635193248026No Hit
GTAATACACTGCAAAATCTTCAGGGTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24800.229105868158811No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24780.22892110536190877No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24630.22753538438514176No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAGTTACTTAGCCTGGTACC24320.22467156103315658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24160.22319345865793846No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGACCCTGAAGATT21860.20194573701417776No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT21690.2003752532405085No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20510.18947424822327474No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20150.18614851787903394No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG20040.18513232249607148No Hit
CATTTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19990.1846704155038158No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18820.1738617918850332No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18620.17201416391601054No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18600.17182940111910824No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18420.17016653594698786No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATAAAGGGTGAGCTACCATATTG18390.16988939175163445No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18150.16767223818880725No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18110.16730271259500273No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17640.16296078686779944No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACTGCTGGTAACACTCTGA17440.16111315889877678No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17430.16102077750032565No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17430.16102077750032565No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17230.159173149531303No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATAC17170.15861886114059617No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA16860.15575503778861105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16630.15363026562423499No Hit
CCCTTTATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16260.15021215388154305No Hit
GACTTCACTCTCACCATCACCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGCTCA15860.1465168979434977No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC15840.14633213514659543No Hit
GTCAGAGTGTTACCAGCAGTTACTTAGCCTGGTACCAGCAGAAACCTGGC15460.14282164200545233No Hit
GAGTGTTACCAGCAGTTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG15420.14245211641164782No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15390.1421749722162944No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14620.13506160453555713No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13650.1261006088857972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13380.1236063111276166No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13090.12092725057253371No Hit
CCCCAGCGCAGCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13020.12028058078337578No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13010.12018819938492466No Hit
GTGATAAAGGGTGAGCTACCATATTGCTGACAGTAATACACTGCAAAATC12810.11834057141590199No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12680.11713961323603723No Hit
TCACCAGACTGGACCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATAT12400.1145529340794055No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12300.11362912009489418No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12140.11215101771967603No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12100.11178149212587152No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12070.11150434793051811No Hit
GTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAAC11750.10854814318008182No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11450.10577670122654782No Hit
GCTTCTCTTCTTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC11330.10466812444513422No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11020.10180430109314909No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAACTGCTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC11000.10161953829624683No Hit
ACCATATTGCTGACAGTAATACACTGCAAAATCTTCAGGGTCCAGTCTGG10920.10088048710863776No Hit
GATAAAGGGTGAGCTACCATATTGCTGACAGTAATACACTGCAAAATCTT10830.10004905452257755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG200.007563188751.3928332
AGTCGGC306.3955353E-445.87634738
GTATCAA25050.040.454711
TGTTAGA9600.039.72698270
GGTATCA8900.038.338081
TATCAAC28000.036.065652
ATCAACG28850.034.8670773
TCAACGC28850.034.739254
AACGCAG29850.033.5754556
CAACGCA29900.033.519315
AGCTGCG4650.032.497370
ACGCAGA31950.031.3686187
CGCAGAG32150.031.2795098
AGAGTAC32850.030.50779311
ATGTTAC450.00485174330.36478226
GTGTACG450.00487595830.33388723
GCTTGTA902.1978667E-730.3296811
TATACGG6200.028.5910825
TACGGCC6300.028.1372537
AATGGTA4350.028.1216470
Produced by FastQC (version 0.11.5)