FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343159
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37980.28276622499644494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34370.2558892878653979No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCAAATACCACAACACTCAGGCTACTATCCCATGCT31650.2356385208303708No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31250.2326604668546315No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29910.22268398603590492No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCAAATACCACAACACTCAGG28000.20846377830174984No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26870.20005077582028635No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25900.1928289949291186No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC23330.1736949981349937No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22980.17108920090622182No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22800.16974907661713914No Hit
GGATAGTAGCCTGAGTGTTGTGGTATTTGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22620.16840895232805647No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21280.15843247150932988No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20910.15567777158177104No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19930.1483815393412098No Hit
GCATGGGATAGTAGCCTGAGTGTTGTGGTATTTGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGG19850.14778592854606193No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19420.14458452052214218No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19190.14287213948609212No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18990.14138311249822247No Hit
ATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG18940.14101085575125508No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18520.13788389907672882No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18320.13639487208885917No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17980.13386352620948078No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17680.1316299857276763No Hit
CTACTATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG17610.13110882628192194No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17420.12969425064344578No Hit
GGCCAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAATATTGGGAA17360.1292475425470849No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17220.12820522365557613No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16750.1247060102340825No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCAAATACCACAACACTCAGGC16730.12455710753529552No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16710.12440820483650857No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG16300.12135569951137579No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16070.1196433184753257No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15970.11889880498139088No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15360.11435727266838848No Hit
CCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTGCTCTGG14960.1113792186926492No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14640.10899677551205776No Hit
GATTATTACTGCGGAGCATGGGATAGTAGCCTGAGTGTTGTGGTATTTGG14630.10892232416266429No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC14440.10750774852418812No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14320.10661433233146633No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14210.10579536748813803No Hit
GTTTGGGGGCTGTTCCTGGGAATTGCTGGTACCAGGATACATAATTATTC14100.10497640264480973No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13920.10363627835572706No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA13910.10356182700633357No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13750.10237060541603785No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13750.10237060541603785No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13660.10170054327149652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.044.214141
CGTATGC1800.044.14636641
TCGTATG1900.041.7598540
TCTCGTA1750.041.29249638
ATAGCGT459.2260045E-538.87212450
GTATCAA41650.038.7160841
TGGTATC4900.038.2658962
ATGCCGT2500.037.4616844
ATCTCGT2000.036.1092337
ACGAGAC2950.035.8302322
TATCTCG2050.035.1915136
AGACCGG2850.034.70244625
TATCAAC46450.034.5684932
GACCGGA2850.033.51829526
GTATGCC2400.033.15480842
ATCAACG48650.033.0052723
TCAACGC48550.033.0018044
GCCTATC2300.032.782533
CAACGCA50000.031.9765745
TAGCGTA552.9751254E-431.85192151
Produced by FastQC (version 0.11.5)