FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343159
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35750.26616357408169844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33780.2514966582511825No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30700.22856564263798998No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30100.22409856167438105No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27750.20660249456691276No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25840.1923822868327577No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCAAATACCACAACACTCAGGCTACTATCCCATGCT25500.1898509409533793No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTG25120.187021789676427No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCAC24380.18151238982130932No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22980.17108920090622182No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22250.16565425240049764No Hit
GGATAGTAGCCTGAGTGTTGTGGTATTTGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22100.1645374821595954No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21630.16103826873810173No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21300.15858137420811683No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20870.1553799661841971No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20410.15195520411209693No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCAAATACCACAACACTCAGG19680.14652025560637275No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19400.14443561782335523No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC19020.1416064665464029No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18140.1350547477997765No Hit
GCATGGGATAGTAGCCTGAGTGTTGTGGTATTTGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGG17980.13386352620948078No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17290.1287263831013305No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17150.12768406420982176No Hit
GGCCAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAATATTGGGAA16880.12567387777619776No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16740.124631558884689No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16600.12358923999318026No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16420.12224911570409758No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15870.11815429148745607No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15800.1176331320417017No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15710.11696306989716035No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15110.11249598893355144No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14560.10840116471690991No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14390.10713549177722073No Hit
CCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTGCTCTGG14160.10542311074117064No Hit
ATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG14070.10475304859662929No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14030.10445524319905536No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13990.10415743780148143No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAG13610.1013282865245291No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13580.10110493247634866No Hit
CTCCTGCTCTGGAAGCAGCTCCAATATTGGGAATAATTATGTATCCTGGTACCAGCAATTCCCAGGAACAGCCCC13490.10043487033180733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)