FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783019
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC44080.5629493026350574No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27220.34762885702645785No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26080.33306982333762014No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC25760.32898307703899904No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC23450.299481877195828No Hit
AGCCAGGACAGGCCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCC22750.2905421196675943No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22570.2882433248746199No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT21470.27419513447310984No Hit
CTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGACTT21280.27176862885830355No Hit
CCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA20780.26538308776670805No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT19930.2545276679109958No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGCTATCTATCCGGTGGTATTCG19510.24916381339405558No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA19320.2467373077792493No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19100.24392766969894727No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18810.2402240558658219No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGACTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT18560.23703128532002415No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGCTATCTATCCGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC17650.2254096005333204No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17650.2254096005333204No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17640.22528188971148846No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17270.22055658930370783No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG17200.21966261355088448No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17090.21825779451073346No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA16980.21685297547058247No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATGCAAGCTGGTA16540.2112336993099784No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16060.20510357986204675No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16010.20446502575288722No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGT16000.20433731493105534No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15770.20139996602892138No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15550.1985903279486194No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15480.19769635219579604No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14790.18888430548939425No Hit
AGATAGCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC14560.18594695658726035No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14220.18160478864497542No Hit
GTGCTATCTATCCGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA14120.1803276804266563No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14020.17905057220833723No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGGATAGATA13930.17790117481185003No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA13700.1749638259097161No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC13380.170877079611095No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13360.17062165796743117No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13240.16908912810544827No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12820.16372527358850808No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12500.15963852728988695No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC12240.15631804592225731No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11980.15299756455462765No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11880.15172045633630857No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11860.15146503469264475No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG11660.14891081825600658No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG11330.14469636113555356No Hit
GAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCT11290.14418551784822592No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGACCTGGAGCCAGAGAATCGGT11290.14418551784822592No Hit
CTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGAC11130.14214214469891537No Hit
GCTTGGTCCCTCCGCCGAATACCACCGGATAGATAGCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCAT11080.14150359058975578No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT11040.14099274730242817No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11000.14048190401510052No Hit
CAACTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGA10960.1399710607277729No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10920.13946021744044523No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10800.13792768757846235No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT10780.13767226593479853No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10710.13677829018197515No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG10450.13345780881434552No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10430.1332023871706817No Hit
GGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGC10420.13307467634884979No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA10350.1321807005960264No Hit
GGACAGGCCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG10340.1320529897741945No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCC10300.13154214648686685No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10040.12822166511923722No Hit
GGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCC9950.12707226772275002No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9820.1254120270389352No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9810.1252843162171033No Hit
ACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATG9800.12515660539527138No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9790.12502889457343946No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9650.12324094306779274No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9620.122857810602297No Hit
GTGATGGTCAAGGAAGCTGTGTTTCCTGACCTGGAGCCAGAGAATCGGTC9570.12221925649313747No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9540.12183612402764173No Hit
GCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAG9350.11940961841283544No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9230.11787708855085255No Hit
GGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGACTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGA9110.11634455868886961No Hit
GGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATT9000.1149397396487186No Hit
GAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGT8950.11430118553955908No Hit
GACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCA8870.1132794989649038No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8830.11276865567757614No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8770.1120023907465847No Hit
AGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATC8770.1120023907465847No Hit
TCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGACCTGGAGCCAGAGAATCGGTCTGGGATC8720.11136383663742513No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8670.1107252825282656No Hit
GAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCA8620.11008672841910604No Hit
CCTCAGAAGTCATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTAGTTGTCATCTATGGTAAAAA8580.1095758851317784No Hit
GTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTC8470.1081710660916274No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGGATAGATAG8390.10714937951697212No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT8290.10587227129865302No Hit
GAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCT8250.1053614280113254No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACT8250.1053614280113254No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8240.1052337171894935No Hit
CTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGGC8230.10510600636766158No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8180.10446745225850204No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8160.10421203061483822No Hit
CAGGGATCCCAGACCGATTCTCTGGCTCCAGGTCAGGAAACACAGCTTCC8120.10370118732751056No Hit
CTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAG8100.10344576568384674No Hit
AGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGCTATCTATC8000.10216865746552767No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7980.10191323582186385No Hit
GAATACCACCGGATAGATAGCACTGCTGTCCCGGGAGTTACAGTAATAGT7940.10140239253453619No Hit
GGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCC7900.10089154924720857No Hit
GTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCC7890.10076383842537666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG100.008711762135.3398670
TACGTAT250.001233766781.2039270
AGGGGGG2850.071.2315170
CCGGCTT252.4386132E-455.7747147
ACCCTTA200.007809761450.9761436
ACCGAAC200.00781962750.9598668
GGTATCA9100.044.8170551
TCGTATG4950.040.39516440
CGTATGC5000.040.0405141
CTAATCT4900.038.3832434
TATACAC6650.038.3279233
TCTCGTA4250.038.01955838
CGAGACT6650.037.8096423
CTCTTAT2250.037.7625161
GTCGTAT557.507455E-637.55088839
CACGAGA6700.037.5274821
TAATCTC4750.037.4934335
AATGTCG557.69609E-637.41532536
GTATGCC5550.037.37946742
TACACAT6650.037.3058475
Produced by FastQC (version 0.11.5)