FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783019
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65330.8343347990278651No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC40080.5118649739022936No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26240.3351131964869307No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24860.3174891030741272No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC22660.28939272227110713No Hit
AGCCAGGACAGGCCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCC21180.2704915206399845No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA20630.26346742543922946No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC19890.25401682462366815No Hit
CCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA19670.2512071865433661No Hit
CTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGACTT19370.24737586188840885No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18620.23779755025101565No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGCTATCTATCCGGTGGTATTCG18270.23332767148689879No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT18040.23039032258476486No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17970.2294963468319415No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGCTATCTATCCGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC17470.22311080574034603No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGACTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT17160.2191517702635568No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17000.2171083971142463No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16460.21021201273532317No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16200.20689153136769348No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16120.20586984479303824No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATGCAAGCTGGTA15930.20344333917823193No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG15440.1971855089084684No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15390.19654695479930884No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15350.1960361115119812No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA14890.19016141370771336No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14710.187862618914739No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGT14280.18237105357596686No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14100.1800722587829925No Hit
GTGCTATCTATCCGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA13810.17636864494986712No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13650.17432527180055657No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13170.16819515235262492No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13170.16819515235262492No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13130.16768430906529727No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13090.16717346577796963No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA12750.1628312978356847No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12590.16078792468637415No Hit
AGATAGCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC12150.15516864852577011No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC12150.15516864852577011No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGGATAGATA12060.15401925112928294No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12020.15350840784195532No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11530.14725057757219173No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC11150.1423975663425792No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG11130.14214214469891537No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10870.1388216633312857No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10870.1388216633312857No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10800.13792768757846235No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10760.1374168442911347No Hit
GGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGC10730.13703371182563895No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA10310.13166985730869876No Hit
GCTTGGTCCCTCCGCCGAATACCACCGGATAGATAGCACTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCAT10260.13103130319953923No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10200.13026503826854774No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10170.12988190580305203No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT10030.12809395429740528No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG9980.12745540018824575No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9980.12745540018824575No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGACCTGGAGCCAGAGAATCGGT9880.12617829196992666No Hit
GAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCT9810.1252843162171033No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG9810.1252843162171033No Hit
GGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGACTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGA9800.12515660539527138No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT9770.12477347292977564No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9650.12324094306779274No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9650.12324094306779274No Hit
ACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATG9580.12234696731496938No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9500.12132528074031408No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTCATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCC9430.12043130498749072No Hit
CTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGAC9320.11902648594733974No Hit
GGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCC9310.11889877512550782No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9140.11672769115436536No Hit
CAACTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATGA8950.11430118553955908No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8860.11315178814307189No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8850.11302407732123998No Hit
GGACAGGCCCCTGTAGTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG8840.11289636649940805No Hit
GTGATGGTCAAGGAAGCTGTGTTTCCTGACCTGGAGCCAGAGAATCGGTC8830.11276865567757614No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC8520.10880962020078697No Hit
GAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGT8510.10868190937895505No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8400.10727709033880403No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT8250.1053614280113254No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8210.10485058472399775No Hit
GTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCC8140.1039566089711744No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8090.10331805486201485No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACT8040.10267950075285528No Hit
GAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCA8010.10229636828735955No Hit
AGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGCTATCTATC7970.10178552500003193No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7890.10076383842537666No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7840.10012528431621709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)