FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678481
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52120.31051885603709545No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47300.2818024153982082No Hit
GAGTTACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT46160.2750105601433677No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36430.21704147976652702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35920.21400301820515094No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35740.21293062000701823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34980.20840271650379122No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA34600.2061387647521777No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTC34170.20357692461219398No Hit
ACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG31870.18987405874716484No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA30310.18057994103001462No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29590.17629034823748377No Hit
GAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC28750.17128582331286443No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28050.16711538587568164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27740.16526847786778642No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27440.1634811475375652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27360.16300452611617292No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATT26550.15817873422457568No Hit
GGTGAGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG26290.15662971460505065No Hit
ACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT25930.1544849182087852No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT25020.1490633495404476No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24160.14393966926048016No Hit
GCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT24060.14334389248373977No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23620.140722474666082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23600.14060331931073392No Hit
CTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTC23300.13881598898051273No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22920.13655203722889922No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22840.13607541580750693No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21760.1296410266187106No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21750.12958144894103657No Hit
GATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT21140.12594721060292013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21100.12570889989222397No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20940.12475565704943933No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20750.1236236811736326No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGGCCAAGGGAC19770.11778506876157668No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA19650.11707013662948822No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACAT19630.11695098127414014No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19370.1154019616546151No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19060.11355505364671988No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19050.11349547596904581No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18990.11313800990300159No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18440.10986123763092939No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17990.10718024213559761No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17910.10670362071420528No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA17630.10503544573933217No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17580.10473755735096196No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17170.10229487256632633No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17130.10205656185563018No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGG17020.10140120740121575No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGTTCACTTTCAGGGAGA16820.10020965384773493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6650.046.431461
CGTATGC3150.046.04920241
TCGTATG3150.042.68358240
GTCGTAT758.949428E-1041.29646739
GTATCAA55850.037.4239921
CTTATAC4900.036.7005351
ATGCCGT3700.035.6509344
GTATGCC4000.035.46055642
CACGAGA5100.035.265521
TCTCCGA5300.035.19745311
ATCTCGT2900.034.3079537
ACGAGAC5400.033.93675222
CCCACGA5350.033.6125719
ATCTCCG5500.033.3008610
TATCAAC63950.032.2034152
CCCTAAG5600.031.58990728
ATCAACG66150.031.184623
TGTCGTA1001.5103069E-830.9218838
TCAACGC66600.030.8720264
TCCGAGC6050.030.83596613
Produced by FastQC (version 0.11.5)