FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678481
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49140.2927647080902316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47000.28001508506798706No Hit
GAGTTACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT43550.25946078627044333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38430.22895701530133497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36150.2153733047916539No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34540.20578129868613348No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTC31770.18927828197042446No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA31640.18850377216066194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31120.18540573292161186No Hit
ACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG28770.1714049786682125No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28430.16937933762729515No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26830.1598469091994488No Hit
GAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC26810.1597277538441007No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA26670.15889366635666416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25750.15341252001065248No Hit
GGTGAGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG25510.15198265574647554No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATT25070.14936123792881778No Hit
ACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT24910.14840799508603314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24730.14733559688790043No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT24690.14709728617720427No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24260.14453544603722057No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23240.1384585229144685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22870.13625414884052903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22080.13154751230427988No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22010.1311304685605616No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21900.13047511410614715No Hit
GCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT21630.1288665168089481No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21350.12719834183407497No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21270.12672172041268265No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20510.12219381690945563No Hit
CTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTC19520.11629562681972569No Hit
GATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT19440.11581900539833338No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19170.1142104081011343No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA19130.11397209739043815No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19130.11397209739043815No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18990.11313800990300159No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18840.11224434473789098No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18510.11027828137464768No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18480.11009954834162557No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA18120.10795475194536011No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGGCCAAGGGAC17810.10610784393746489No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACAT17690.10539291180537641No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17390.10360558147515522No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17300.10306938237608886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6450.044.643041
TACGTAT700.001246275840.06035270
GTATCAA54300.035.8725781
TATCAAC62550.030.855662
ATCAACG63450.030.3483333
TCAACGC64550.029.8196134
CAACGCA64900.029.6570285
AACGCAG65700.029.5009776
ACGCAGA68700.028.1142527
CGCAGAG69450.027.7619368
GTACGTA650.00835547827.16709569
AGAGTAC71250.027.01230211
GCAGAGT72650.026.632239
GACAGGT3700.025.6360361
CAGAGTA80050.024.42309610
GTCTACT6350.024.18406767
GAGTTTA7650.023.9126031
GTCATAT1001.8015246E-523.7133331
TGTCTAC6300.023.38644666
CGAAATT1502.4907422E-823.32389548
Produced by FastQC (version 0.11.5)