FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705311
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC152030.8915089388387221No Hit
CCCATATACAGCACACAGTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC117190.6872060286950591No Hit
GTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGATGGTGAGGGCAG73160.4290126551696435No Hit
GATTATTACTGTGTGCTGTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT72390.4244973497502802No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71540.4195129216899439No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG68040.3989888061473831No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTACCCATATACAGCACA63110.3700791233974331No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT58650.3439255361631984No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56320.33026233924486503No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTACCCATATACAGCACACAGTAATAAT55820.3273303227387849No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA54740.3209971670856518No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT54200.3178305892590853No Hit
CTCATCTACAGCACAGACACTCGCTCTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG52700.30903453974084494No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG51490.301939059796131No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG49940.29284980862728266No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48670.2854024867018391No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48270.28305687349697506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT47340.277603322795666No Hit
CCCCAGAAGAGCGAGTGTCTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG46700.27385034166788347No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG45980.26962823789912804No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45340.2658752567713455No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT45260.26540613413037273No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC41000.24042535349857005No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40170.23555820609847702No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG40090.23508908345750423No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT39590.23215706695142413No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG39530.2318052249706945No Hit
CTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAGACA38780.22740720021157432No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG38070.22324373677294054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37480.219783957295766No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAGACACTCGCTCT37250.21843522970296914No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36390.21339216131251132No Hit
CTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCT36090.21163295140886323No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34920.20477203278463577No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC34400.20172273561831244No Hit
CATCTACAGCACAGACACTCGCTCTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC33460.1962105445868818No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT33400.19585870260615218No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG33230.19486181699408495No Hit
GTGCTGTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG32560.19093291487593758No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32230.1889977839819247No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG32010.18770769671924947No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA31540.18495160120353413No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA31530.18489296087341253No Hit
CCCAAAAGCCATTACCCATATACAGCACACAGTAATAATCAGATTCATCA31240.18319239129988607No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG30950.1814918217263596No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA30770.18043629578417075No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC30750.18031901512392753No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30750.18031901512392753No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG30350.17797340191906344No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC29740.17439634178164568No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC29600.17357537715994326No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGA29600.17357537715994326No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29490.17293033352860562No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC29210.17128840428520076No Hit
CAGTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGATGGTGAGGGC28640.16794590546826943No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC28480.1670076601863238No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC28330.16612805523449975No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT27530.16143682882477156No Hit
GTGCTGTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGA27020.15844617198856983No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC26610.15604191845358412No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC26570.1558073571330977No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC26020.1525821389764096No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC25870.15170253402458556No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT25570.14994332412093747No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC25420.14906371916911343No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25370.14877051751850542No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCGGGGACCCCAGAAGAGCGAGTGT25150.14748043025583016No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25060.14695266728473574No Hit
CACCATCACGGGGGCCCAGGTGGATGATGAATCTGATTATTACTGTGTGC24640.14448977341962843No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT24500.143668808797926No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG24380.14296512483646676No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23870.13997446800026506No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23570.138215258096617No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA23330.13680789017369852No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23120.13557644324114487No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23060.13522460126041527No Hit
CTGTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG22950.13457955762907764No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG22410.1314129798025111No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTAC22260.13053337485068706No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC22070.1294192085783766No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG21590.1266044727325397No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC21560.12642855174217488No Hit
AGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAAT21460.12584214844095887No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC21360.12525574513974283No Hit
CTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAGA20800.1219718866529331No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT20520.12032995740952823No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20450.11991947509867702No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA20360.11939171212758261No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC20340.11927443146733938No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20310.11909851047697458No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20280.11892258948660978No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCGGGGACCCCA20090.11780842321429932No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC19850.11640105529138088No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19630.1151109680287056No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTA19420.11387952109615197No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG19340.11341039845517914No Hit
TTATTACTGTGTGCTGTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAG19250.11288263548408471No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA19140.11223759185274709No Hit
GCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTACCCATATACAGCACACAGTAATAATCAGATTCATCATCCA19130.11217895152262548No Hit
GTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT18860.11059566260934223No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT18640.10930557534666697No Hit
GGATGGAGCCAGAGAAGCGATCGGGGACCCCAGAAGAGCGAGTGTCTGTGCTGTAGATGAGCGTGCGTGGAGC18540.10871917204545095No Hit
CCATCACGGGGGCCCAGGTGGATGATGAATCTGATTATTACTGTGTGCTG18530.10866053171532934No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18530.10866053171532934No Hit
CTACAGCACAGACACTCGCTCTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCT18300.1073118041225325No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17840.10461434893693877No Hit
GCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCGGGGACCCCAGAAGA17700.10379338431523635No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG17510.10267921804292589No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17460.10238601639231788No Hit
CCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGC17310.10150641144049384No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT17270.10127185012000745No Hit
GAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTC17250.10115456945976424No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17130.100450885498305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG750.050.54385840
GGTATCA16250.043.5204351
GTGGTAT4850.042.265481
TGGTATC4850.042.2642362
ACGAGAC2900.041.2270922
CACGAGA2950.040.52713421
TATACAC3050.040.324243
CGTATGC602.913348E-740.2470141
GTATCAA41600.040.159821
AATGGTA3200.038.26006370
TCTCGTA901.2732926E-1038.2094238
AGCGCTT3400.038.12767870
CGAGACT3150.037.95509323
TTATACA3250.037.8427472
AGGGGGG2300.037.57510470
CGAGCCC3350.035.68702715
CTTATAC3450.035.650011
CACCGTA1459.1605034E-734.7677770
TATCAAC48550.034.409882
ATACACA4100.032.4970784
Produced by FastQC (version 0.11.5)