FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705311
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC141420.8292915485797019No Hit
CCCATATACAGCACACAGTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC97860.5738542705700016No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG70540.41364888867778365No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC68470.40151034034261196No Hit
GATTATTACTGTGTGCTGTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT66220.38831626606525144No Hit
GTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGATGGTGAGGGCAG65780.38573609153990096No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG57590.33770966117030854No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTACCCATATACAGCACA56190.3295000149532842No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT55250.32398782392185355No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT52700.30903453974084494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50200.2943744572104443No Hit
CTCATCTACAGCACAGACACTCGCTCTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG49380.2895659501404729No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTACCCATATACAGCACACAGTAATAAT48950.287044415945244No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT48010.28153222491381336No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG46870.2748472272799507No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA45700.2679863086557232No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG45650.26769310700511517No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44470.2607735480507661No Hit
CCCCAGAAGAGCGAGTGTCTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG41220.24171544076124532No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40930.24001487118771883No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40920.23995623085759726No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39150.22957689242607363No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39040.228931848794736No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38540.22599983228865586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37150.2178488264017531No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG36650.214916809895673No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC35700.20934597853412076No Hit
CTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAGACA35620.20887685589314792No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35430.2077626896208375No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35010.20529979575573015No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG34680.2033646648617173No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34320.20125361297733962No Hit
CTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCT34220.2006672096761236No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG34040.19961168373393476No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAGACACTCGCTCT33300.19527229930493617No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32910.19298532643019367No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32440.19022923091447835No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT32360.18976010827350553No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31910.18712129341803344No Hit
GTGCTGTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG31880.18694537242766862No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA31420.1842479172420749No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG31250.18325103163000767No Hit
CATCTACAGCACAGACACTCGCTCTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC30700.18002581347331953No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG29910.17539322739371294No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA29810.17480682409249693No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29380.17228528989726802No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG29270.17164024626593039No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC29030.17023287834301193No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC28870.1692946330610663No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28680.16818046678875584No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC28420.16665581820559416No Hit
GTGCTGTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGA28080.1646620469814597No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27660.16219915311635238No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC26950.1580356896777186No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC26810.15721472505601616No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26750.15686288307528656No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC26550.15569007647285452No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT26060.15281670029689598No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26050.15275805996677438No Hit
CCCAAAAGCCATTACCCATATACAGCACACAGTAATAATCAGATTCATCA25920.15199573567519356No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25530.14970876280045106No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGA25480.14941556114984306No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24600.14425521209914205No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC23880.14003310833038665No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCGGGGACCCCAGAAGAGCGAGTGT23590.1383325387568602No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG23490.13774613545564418No Hit
CAGTAATAATCAGATTCATCATCCACCTGGGCCCCCGTGATGGTGAGGGC23470.13762885479540093No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC23240.1362801272026041No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22890.134227715648348No Hit
AACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC22890.134227715648348No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC22730.13328947036640237No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT22120.12971241022898464No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG22070.1294192085783766No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21860.12818776164582296No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC21710.12730815669399892No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA21470.12590078877108046No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA21290.12484526282889163No Hit
CACCATCACGGGGGCCCAGGTGGATGATGAATCTGATTATTACTGTGTGC21190.1242588595276756No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG21110.12378973688670278No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21100.12373109655658117No Hit
CTGTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG20690.12132684302159547No Hit
CTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAGA20190.11839482651551535No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20070.11769114255405612No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC19580.1148177663780976No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC19490.11429000340700318No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC19430.11393816142627355No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19110.11206167086238229No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCGGGGACCCCA19080.11188574987201748No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT19070.11182710954189588No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTA18730.10983333831776139No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18530.10866053171532934No Hit
GCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTACCCATATACAGCACACAGTAATAATCAGATTCATCATCCA18390.1078395670936269No Hit
AGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAAT18250.10701860247192448No Hit
TTATTACTGTGTGCTGTATATGGGTAATGGCTTTTGGGTGTTCGGCGGAG18190.10666676049119485No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18050.10584579586949243No Hit
GTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT17990.1054939538887628No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17900.1049661909176684No Hit
GGATGGAGCCAGAGAAGCGATCGGGGACCCCAGAAGAGCGAGTGTCTGTGCTGTAGATGAGCGTGCGTGGAGC17850.10467298926706038No Hit
CTACAGCACAGACACTCGCTCTTCTGGGGTCCCCGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCT17650.10350018266462833No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAAGCCATTAC17480.10250329705256107No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT17460.10238601639231788No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC17290.10138913078025064No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG17120.1003922451681834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT406.424622E-573.0336870
GTGGTAT5150.048.4281041
TGGTATC5650.043.518572
GGTATCA17700.043.044081
GTATCAA45450.039.2390441
TATCAAC50450.035.0638122
ATCAACG53200.033.3649983
TCAACGC54100.032.859544
AGTCGGG1352.0368976E-532.45941570
AACGCAG55200.032.4491276
CAACGCA55100.032.2603385
TTTCGAT1153.0284168E-431.75377570
ACGCAGA58050.030.8560187
CGCAGAG58450.030.7615978
CGATAGC450.004873715330.33773414
AGCGCTT3151.8189894E-1230.14088470
AGAGTAC59600.029.93555311
GCAGAGT60350.029.9053219
TCCGAGA2701.5825208E-1029.75446370
CAGAGTA60450.029.74122610
Produced by FastQC (version 0.11.5)