FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011278
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATACGTCACCTCACACTTTTGGCCAGGGGA44170.43677406212732806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37280.36864245044389377No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33020.3265175352375904No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29220.2889413197953481No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26560.2626379689857784No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26230.2593747713289521No Hit
GTATGATACGTCACCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25130.24849744580619768No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24800.2452342481493714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24620.24345432215473883No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24510.2423665896024634No Hit
GTGTATTACTGTCAGCAGTATGATACGTCACCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA23690.23425803784913746No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21780.21537104535053667No Hit
CAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCA21030.20795468703956776No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20060.19836286362404798No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19880.19658293762941542No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19690.19470412685730334No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17030.16840077604773365No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17000.1681041217152949No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16940.1675108130504174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16870.1668186196080603No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15410.15238144209604085No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA14990.14822828144189826No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14940.14773385755450036No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14940.14773385755450036No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14760.14595393155986783No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14690.14526173811751072No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14410.1424929643480823No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.1424929643480823No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14410.1424929643480823No Hit
GTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCAGTGCCA14280.1412074622408477No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14220.1406141535759702No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT14150.13992196013361313No Hit
CCTCTATGGTGCTTCCAGCAGGGCCACTGGCGTCCCATACAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCA14060.13903199713629685No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13290.13141786927036878No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13140.129934597608175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13090.12944017372077707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13080.1293412889432975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12580.12439705006931823No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12440.12301266318460403No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12400.1226171240746857No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12300.12162827629988984No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12160.12024388941517565No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12110.11974946552777772No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12090.11955169597281856No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12020.11885950253046144No Hit
GTATCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11830.11698069175834934No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11640.11510188098623722No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11590.11460745709883928No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA11260.11134425944201297No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAACAACTACTTAGCCTGGTATCAGCACAAACCTGGCCGGGCTCCCA11170.11045429644469672No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10840.10719109878787039No Hit
GTCCCAGACCCAGTGCCACTGAACCTGTATGGGACGCCAGTGGCCCTGCT10810.10689444445543164No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10780.10659779012299289No Hit
CCATAGAGGAGGAGAGTGGGAGCCCGGCCAGGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGTTAACACTCTGA10630.10511451846079911No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10560.10442232501844202No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10370.10254351424632989No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10150.10036804914177902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA150.002538957267.7068122
TCGTATG2100.047.64571440
GTGGTAT4450.042.5788651
CCCACGA3900.042.51483519
CGAGACG4000.041.4704223
GAGACGC4050.040.96857524
ATCTCGT2100.040.8678237
TTATACA4100.040.434942
CGTATGC2400.040.3378441
CGCTCAT4200.038.81057728
GCTTGAC702.5984264E-838.668561
ACGCTCA4300.038.65746727
ACGAGAC4300.038.57713722
TCTCCGA4300.038.5542511
ATCTCCG4300.038.5523410
CGAGCCC4400.037.67987415
TCCGAGC4500.037.5925713
GACGCTC4450.037.3321926
GTATCAA41400.037.0223431
CACGAGA4400.036.9273321
Produced by FastQC (version 0.11.5)