FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252468
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG55520.4432847785332639No Hit
GATTATTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTTGGGTGTTCGG52550.41957159783723014No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43900.3505079570895225No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35530.28367990240070007No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32850.26228215012279754No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC30080.24016581661168188No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29640.23665275280486206No Hit
GCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCACG27190.21709137478961538No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26980.21541468524545138No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26430.2110233554869266No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26370.21054430133145116No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26250.20958619302050033No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24220.19337819409358165No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24080.19226040106413897No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23690.18914654905354866No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23620.18858765253882734No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22710.18132199784745No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22210.17732987988515475No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21900.1748547667485317No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTC21620.17261918068964638No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21400.17086264878623647No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCCGCTGGTACCAGTTTACAGTCTTACTTCCGATGTTTGAG20610.16455510240580998No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAT20600.16447526004656407No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20460.1633574670171214No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20320.16223967398767875No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19790.15800802894764576No Hit
GACCTAGGACGGCCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG19740.15760881715141625No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19380.15473449221856367No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19260.1537763839076128No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18190.145233251468301No Hit
CCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCCGCTGGTACCAGTTTA18120.14467435495357966No Hit
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTCCATTTGCAGATACA17940.1432371924871534No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17460.1394047592433499No Hit
GTCTATTACTGTGCGAGGAGTGGTATCAGGCCATTTGACTACTGGGGCCA17430.13916523216561222No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17220.13748854262144822No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAGGTCTGGCACCTCAGCCTC17190.1372490155437105No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16870.13469406004784154No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16620.13269800106669394No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCCGCTGG16080.12838651366741508No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16020.12790745951193963No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15850.12655013940475923No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAAGCGGTCAGGGACC15700.12535250401607068No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC15690.12527266165682477No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15690.12527266165682477No Hit
GTCTTACTTCCGATGTTTGAGCTGCTTCCAGAACAAGAGATGATGACCCT15420.12311691795718534No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15380.12279754852020171No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15340.12247817908321809No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15280.12199912492774267No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14910.11904495763564418No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14690.11728842573223427No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14670.11712874101374247No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAG14650.11696905629525066No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14270.11393504664390626No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13720.10954371688538149No Hit
TTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAG13570.10834608149669293No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13500.1077871849819716No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13490.10770734262272569No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13050.10419427881590587No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAAGCGGTC12830.10243774691249598No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTCCGCCGAACAC12820.10235790455325007No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12770.10195869275702053No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG12760.10187885039777464No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12600.10060137264984016No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12550.10020216085361064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG7400.040.83477870
CGTATGC1750.039.47162641
TCGTATG1750.039.42553740
TTGCAAT700.001378813939.24381370
GTATCAA28650.038.806531
CACGAGA2500.035.5735721
TTATACA2650.034.853522
ACGAGAC2650.034.8521322
GTGTATA601.4226953E-534.2094541
AGCTAGT400.00270688734.2080882
TATAAGA1300.034.2080842
CTTATAC2750.033.5874631
ATCTCGT1950.033.53447737
GAGTTTA4750.033.1291541
TATCAAC33800.033.0948072
CGAGACA2800.032.98636623
TATACAC2950.032.4686933
ATCAACG34250.032.4602283
ATGCCGT2150.032.24114244
GATCTCG2250.032.1134736
Produced by FastQC (version 0.11.5)