FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763532
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA25630.33567682821414163No Hit
ATATTACTGTGCGAGAGATCTGAAACCGGTGACTACGTGGTTCTTCGATCTCTGGGGCCGTGGCACCCTGGTCA23930.3134118805760597No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20490.2683581041789997No Hit
CTATATTACTGTGCGAGAGATCTGAAACCGGTGACTACGTGGTTCTTCGA20150.26390511465138333No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18640.24412860233755757No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18470.24190210757374936No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15390.2015632612647538No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15030.1968483311766894No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15020.19671736089646538No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15020.19671736089646538No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14050.18401324371473626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13870.18165577867070404No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13780.18047704614868793No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13750.18008413530801592No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTCGCAGCTGGTTAGCCTGGTATCAGCAGAAA13540.17733375942331164No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13450.17615502690129556No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATA13400.17550017550017552No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13010.17039233457143904No Hit
GCCCTATATTACTGTGCGAGAGATCTGAAACCGGTGACTACGTGGTTCTT12890.1688206912087509No Hit
GGCTAACGGTTTCCCGCTCACCTTCGGCCAAGGGACACGACTGCAGATTAAACGAACTGTGGCTGCACCAT12750.16698710728561475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12190.1596527715930701No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11830.15493784150500567No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACGGTTTCCCGCTCACCTT11800.15454493066433367No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11740.15375910898298958No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11390.14917514917514918No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11230.147079624691565No Hit
ATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACAGGG10960.14354342712551668No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTTACGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG10870.14236469460350057No Hit
CCGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC10690.14000722955946837No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10610.13895946731767628No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10510.13764976451543615No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10440.13673297255386807No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10320.13516132919117993No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10220.13385162638893983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10080.13201804246580365No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT10040.1314941613449076No Hit
CCTTCGGCCAAGGGACACGACTGCAGATTAAACGAACTGTGGCTGCACCA10030.13136319106468358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10020.1312322207844596No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9830.12874378546020337No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9800.12835087461953132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9630.12612437985572314No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9630.12612437985572314No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9550.12507661761393105No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9530.12481467705348302No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9490.12429079593258698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9420.1233740039710189No Hit
AGTCAGGGCATTCGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTGAGCTCCTCATCTCTT9410.12324303369079488No Hit
CCTCATCTCTTCTGCATCCAACTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTT9390.12298109313034686No Hit
GTGCAGCCACAGTTCGTTTAATCTGCAGTCGTGTCCCTTGGCCGAAGGTG9300.12180236060833076No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9280.12154042004788274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9160.1199687766851946No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9110.11931392528407454No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9060.11865907388295448No Hit
GGACACGACTGCAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9000.1178732522016104No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8870.11617063855869827No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACAGTGACCAGGGTGCCACGG8810.11538481687735419No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGCGGGAAACCGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG8730.1143370546355621No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8690.11381317351466605No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8680.11368220323444203No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8550.11197958959152989No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGCGTAACTGC8460.11080085706951379No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8460.11080085706951379No Hit
GATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACGGTTTC8340.10922921370682565No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8330.10909824342660163No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8290.1085743623057056No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT8280.10844339202548157No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8240.10791951090458553No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8210.10752660006391349No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8170.10700271894301745No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8170.10700271894301745No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8160.10687174866279345No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGGGGTACAACTGTTGGAAT8070.10569301614077733No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8060.10556204586055333No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8030.10516913501988129No Hit
ATCTTGATGGGACCCCACTTTGCAAGTTGGATGCAGAAGAGATGAGGAGCTCAGGGGCTTTTCCTGGTTTCTG7960.10425234305831321No Hit
GTATGTGTCGGCGGCACTATAAGTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT7920.10372846193741717No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7710.10097808605271293No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG7680.10058517521204088No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA7640.10006129409114485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG100.008749181135.1456870
GTACGGT150.002409596568.604111
AACGTCG150.002410848668.5951319
AATCTCG700.054.1408336
GTTAGAT200.006389085753.6310766
GCTTGTA453.1670425E-853.3587531
TCGTATG707.2759576E-1249.34229340
CTTATAC1200.042.877571
CGTAGAG1150.041.80557628
ATGCCGT856.366463E-1140.7315444
TATGCCG856.366463E-1140.69925343
CGTATGC856.548362E-1140.653641
ATCTCGT856.730261E-1140.5599337
CGAGACG1100.040.53879523
GTATGCC955.456968E-1240.0381242
GTGGTAT1150.038.776241
TTATACA1350.038.1133962
TACACAT1350.038.1133965
CCGTCTT753.8317012E-836.99841747
GTATCAA13350.036.486081
Produced by FastQC (version 0.11.5)