FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763532
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTACTGTGCGAGAGATCTGAAACCGGTGACTACGTGGTTCTTCGATCTCTGGGGCCGTGGCACCCTGGTCA22700.29730253610850627No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21390.28014542939916076No Hit
CTATATTACTGTGCGAGAGATCTGAAACCGGTGACTACGTGGTTCTTCGA17360.22736440646888406No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA16920.22160171413902757No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16660.21819648685320325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14970.19606250949534532No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14650.19187146052817697No Hit
GCCCTATATTACTGTGCGAGAGATCTGAAACCGGTGACTACGTGGTTCTT14460.18938302520392072No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGTTACGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG14280.18702556015988853No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13450.17615502690129556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12900.16895166148897492No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12880.16868972092852688No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12030.15755724710948593No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11700.15323522786209354No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11610.15205649534007742No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11320.1482583572135811No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10930.14315051628484463No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTCGCAGCTGGTTAGCCTGGTATCAGCAGAAA10800.1414479026419325No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10800.1414479026419325No Hit
CCGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC10720.1404001404001404No Hit
GGCTAACGGTTTCCCGCTCACCTTCGGCCAAGGGACACGACTGCAGATTAAACGAACTGTGGCTGCACCAT10570.13843558619678023No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10350.13555424003185199No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9960.1304463991031155No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9930.13005348826244348No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9880.12939863686132344No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACGGTTTCCCGCTCACCTT9870.12926766658109942No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9730.12743408265796324No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9620.12599340957549912No Hit
GTCGTGTCCCTTGGCCGAAGGTGAGCGGGAAACCGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG9590.1256004987348271No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9360.12258818228967482No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9330.1221952714490028No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9230.12088556864676267No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGCGTAACTGC9200.12049265780609064No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9180.12023071724564262No Hit
GGACACGACTGCAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9020.11813519276205843No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8990.1177422819213864No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8900.11656354939937029No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATA8880.11630160883892228No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8840.11577772771802623No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT8790.11512287631690615No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8730.1143370546355621No Hit
GTGCAGCCACAGTTCGTTTAATCTGCAGTCGTGTCCCTTGGCCGAAGGTG8710.11407511407511407No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8520.11158667875085786No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8430.11040794622884176No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8370.10962212454749767No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGGGGTACAACTGTTGGAAT8350.10936018398704965No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8300.10870533258592961No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8280.10844339202548157No Hit
CCTCATCTCTTCTGCATCCAACTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTT8280.10844339202548157No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8240.10791951090458553No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT8150.10674077838256943No Hit
GATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACGGTTTC8140.10660980810234541No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8110.10621689726167337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8100.10608592698144936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8020.10503816473965727No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8000.10477622417920925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7930.10385943221764117No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7930.10385943221764117No Hit
GTGTGGGGTACAACTGTTGGAATCTGGGGGAACCTGGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC7900.10346652137696913No Hit
CCTTCGGCCAAGGGACACGACTGCAGATTAAACGAACTGTGGCTGCACCA7720.10110905633293693No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7640.10006129409114485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1950.049.241731
GTATCAA14850.037.410921
GACAGGA1500.034.2933431
CGTTGGT400.00268804434.25515714
TAGGATA400.00268804434.2551575
TATCAAC16850.032.953172
CCGTGCA553.0171E-431.77373956
CTTAGCA900.003947533731.66657870
ATCAACG17550.031.4373153
TAATGTG450.004432934330.92916551
TCAACGC17750.030.8799114
TAGTTCG450.00452459730.80006447
CGGTACC450.00454958630.7654145
CAACGCA18000.030.6393345
AATTGAG450.00467617630.5933135
AACGCAG17950.030.5318476
CCGTTGG450.004785570330.4490313
CGCAGAG18450.029.518778
AGTCTTA500.005854679329.21843767
GCAGAGT18950.028.7399149
Produced by FastQC (version 0.11.5)