FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933344
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACTGCAGTGTCCTCAGCTCTCAGGCTGTTCATCTGCAGATACA69400.35896353675290066No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42480.21972292566661702No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36550.18905068109969048No Hit
CTCCTGCACTGGGAGCAGCTCCAACTTCGGGGCAGATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC35470.18346450502342057No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34020.17596454640250261No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC33380.1726542198387871No Hit
GCTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC33040.17089560885181324No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG32800.16965423639041993No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGCTGTCAAAGGA32570.16846458778158466No Hit
ATGCTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG31870.1648439181025208No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31400.16241289703229222No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG30890.15977498055183145No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29340.15175778340533294No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28950.1497405531555688No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28230.14601643577138887No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27670.14311990002813776No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27260.14099922207325755No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26760.13841302944535477No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA26530.13722338083651953No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26370.13639579919559064No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26240.13572338911233595No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26150.13525787443931345No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTAACTATGCTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGG25830.1336027111574557No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25340.131068242382111No Hit
GTTTATTACTGTGCGAGAGATAAAGGGAGCAGTGGCCCGGGAGGGAGCTACTTTGACTACTGGGGCCAGGGAAGC24690.12770619196583743No Hit
GTCATCATCTCCTGCACTGGGAGCAGCTCCAACTTCGGGGCAGATTATGA24670.12760274426072132No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT24430.12636137179932802No Hit
GGGCTGGAGTGGGTGGCAGATATATTAGCTGCTGGAAGCGTGAAATATTA24090.12460276081235414No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGTATAGCATAGTTAC24020.12424069384444775No Hit
GATTATTACTGCCAGTCCTTTGACAGCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA23870.12346483605607693No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23770.12294759753049637No Hit
GATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATTTATCGTAGCAACAGTCGG23600.12206829203700945No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT23450.12129243424863863No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGACTGTTG23140.11968899481933892No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23070.11932692785143253No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT22660.11720624989655229No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22440.11606832514027508No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21890.11322351324958206No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21410.11074076832679543No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT21350.11043042521144712No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20940.10830974725656685No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20770.10743044176307992No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20660.10686147938494131No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20530.10618906930168662No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20140.10417183905192247No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTTT19850.10267184732773889No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19710.10194771339192611No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19620.10148219871890363No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCATCATCTCCTGCACTGGGAGCAG19480.10075806478309086No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19420.10044772166774253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5550.047.3392261
GGTATCA18800.043.0144041
TGGTATC6550.040.1108252
GTATCAA47900.038.6088751
AGGGGGG3350.037.0537770
CGTATGC3150.035.10406541
TCGTATG3000.034.50223540
TCTCGTA2700.034.41581338
ATCTCGT2800.034.37545437
TATCAAC54050.034.2780652
TCTGGAG31200.033.15441570
ATCAACG56750.032.587093
CTTATAC4450.032.2042121
TCAACGC57450.032.1306424
CAACGCA58150.031.9198865
CACGAGA4600.031.89006421
AACGCAG58450.031.8144286
CCCTAAG4900.031.37631628
GCGTATG553.2565917E-431.3656740
ATGCCGT3300.030.48761744
Produced by FastQC (version 0.11.5)