FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933344
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACTGCAGTGTCCTCAGCTCTCAGGCTGTTCATCTGCAGATACA60540.3131362033864641No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46580.24092970521541948No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40170.20777471572570633No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38750.20042992866246256No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCATCATCTCCTG34090.176326613370409No Hit
CTCCTGCACTGGGAGCAGCTCCAACTTCGGGGCAGATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGC33540.17348180147971598No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31800.16448185113461444No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30700.1587922273532284No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27660.1430681761755797No Hit
ATGCTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG27420.1418268037141864No Hit
GCTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC27200.1406888789579092No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27150.14043025969511894No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26450.13680959001605508No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25850.13370615886257178No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGTATAGCATAGTTAC25570.13225789099094626No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25540.1321027194332721No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC25480.13179237631792376No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGCTGTCAAAGGA25310.13091307082443682No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTAACTATGCTATACACTGGGTCCGCCAGGCTCCAGGCAAGGGG25280.13075789926676268No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAT24990.12925790754257907No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24280.1255855140109572No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA24250.12543034245328302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24190.1251199993379347No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24110.12470620851747025No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23690.12253380671003195No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23610.1221200158895675No Hit
GTCATCATCTCCTGCACTGGGAGCAGCTCCAACTTCGGGGCAGATTATGA23420.12113726269096448No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22960.11875796547329394No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT22590.1168441829286459No Hit
GATTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCATTTATCGTAGCAACAGTCGG22510.11643039210818147No Hit
GTTTATTACTGTGCGAGAGATAAAGGGAGCAGTGGCCCGGGAGGGAGCTACTTTGACTACTGGGGCCAGGGAAGC22460.11617177284539121No Hit
GATTATTACTGCCAGTCCTTTGACAGCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA22440.11606832514027508No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT22370.1157062581723687No Hit
GGGCTGGAGTGGGTGGCAGATATATTAGCTGCTGGAAGCGTGAAATATTA22130.11446488571097539No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT21800.11275799857655958No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21710.11229248390353709No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21620.11182696923051458No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21570.1115683499677243No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG21460.1109993875895857No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAT21210.10970629127563433No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20210.10453390601982886No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20150.10422356290448052No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT20080.10386149593657414No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19890.1028787427379711No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19690.10184426568681001No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19560.1011718556035553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.043.398181
GTGGTAT5850.043.1332321
GTATCAA44700.038.2941441
TGGTATC6450.038.049722
GGATGGG15950.034.7791270
TATCAAC50100.033.9501382
ATCAACG54100.031.3623393
TCAACGC54300.031.2379344
AACGCAG55300.030.857036
CAACGCA54950.030.805645
TCTGGAG30500.030.47041770
CGCAGAG58850.029.2271488
AGAGTAC59300.029.00010511
CAGAGTA59850.028.6220310
GCAGAGT61350.027.924399
AATGGTA6250.027.66430570
GACAGGT4000.027.2788521
TCCGAGA9750.025.86139570
GAGTACG52750.024.98920812
AGTACGG52500.024.91808113
Produced by FastQC (version 0.11.5)