FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127909
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39590.3510034940762065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39230.3478117472242885No Hit
GAGTTACAGTATTCCGGGCGGTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGAACTGTGGCTGCACCAT33980.30126543896715074No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31140.2760861026909086No Hit
GTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGAACTGTGGCTGCACCA28940.25658098304029847No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28360.2514387242233194No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27570.2444346130760549No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26650.23627792667670885No Hit
CTACTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA26210.23237690274658684No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGCAGATCTGGGACAGACTTCA25800.22874185772079134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24950.22120578876487373No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22870.20276458473156966No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22740.20161200947948812No Hit
GAATACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAGT22680.20108005167083515No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22590.20028211495785564No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAGTCTGTC22470.19921819934054963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21170.18769244681973457No Hit
GACTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATT20980.18600791375900005No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20930.18556461558512258No Hit
GGCCAAAACCGCCCGGAATACTGTAACTCTGTTGACAGTAGTAAGTTGCA20760.18405740179393903No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20460.18139761275067404No Hit
ACAGTATTCCGGGCGGTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGA20360.18051101640291903No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19850.1759893750293685No Hit
GAGTGAAGTCTGTCCCAGATCTGCTGCCACTGAACCTTGATGGGACCCCA19240.17058113730806299No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19140.16969454096030795No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT19110.16942856205598147No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAGTTATTTAAATTGGTATCAGCAAAAA18740.16614815556928794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18720.16597083629973697No Hit
ACTTACTACTGTCAACAGAGTTACAGTATTCCGGGCGGTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGAA18720.16597083629973697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17780.1576368306308399No Hit
GTATTAGTAGTTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCT17500.1551543608571259No Hit
GATCTGGGACAGACTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGAT17470.1548883819527994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17430.15453374341369738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17380.15409044523981988No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGGTTGGTCCCCTGGCCAAAACCGCCCGGAATA17240.15284921035296287No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17050.15116467729222838No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16190.1435399487015353No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16060.14238737344945382No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15950.1414121174669233No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15840.1404368614843928No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGGTTGGTCCCCTGGCCAAAACCG15660.13884098805843378No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15470.13715645499769927No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15370.1362698586499443No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAGTCTGTCCCAGATCTGCTGCCA15240.13511728339786278No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14920.13228017508504675No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14850.13165955764161824No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14840.13157089800684274No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14550.12899976859835324No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT14510.12864513005925124No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGCAGATCTGGGACAGACTTCACTCTTA13780.12217297672063969No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13650.12102040146855818No Hit
CTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA13530.1199564858512522No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13510.1197791665817012No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13430.11906988950349717No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13270.11765133534708917No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13230.11729669680798716No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT13000.11525752520815066No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12730.11286371506921214No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATTCCGGGCGGTTTTGGCCAGGGGA12390.10984928748684512No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12250.10860805259998811No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12190.10807609479133512No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11940.1058596039219476No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11840.1049730075741926No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGCAGATCTGGGACAGA11800.1046183690350906No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11790.10452970940031509No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11790.10452970940031509No Hit
GTCCCAGATCTGCTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT11720.10390909195688658No Hit
GAGTATTAGTAGTTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC11700.10373177268733558No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11410.10116064327884608No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11410.10116064327884608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT150.002412923168.5823737
ATTACGA200.002674238566.8251769
TCGTGAC200.007702637551.15602525
GTATCAA26950.038.05981
CGTATGC557.471175E-637.57959441
GTGGTAT2950.036.964661
TCGTATG709.959076E-734.4001640
ATCAACG30600.033.4071433
TATCAAC30500.033.4049532
TCAACGC30950.033.0293584
CAACGCA31500.032.344485
AACGCAG31650.032.298856
ACGCAGA33450.030.4535267
GTTAACC450.004825704730.39831533
TAGGATA450.00491137430.2891454
CGCAGAG34350.029.556438
AGAGTAC34900.029.28588111
CACGAGA1750.029.21515821
ATGCCGT953.1432864E-729.11582444
GCAGAGT34950.029.0490259
Produced by FastQC (version 0.11.5)