FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127909
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39750.35242204823261447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33480.2968324572283757No Hit
GAGTTACAGTATTCCGGGCGGTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGAACTGTGGCTGCACCAT32560.2886757708290296No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31070.2754654852474801No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28850.25578304632731896No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26850.23805111937221884No Hit
GTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGAACTGTGGCTGCACCA25640.2273233035643833No Hit
CTACTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA25010.22173774657352677No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGCAGATCTGGGACAGACTTCA24680.21881197862593524No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24300.21544291250446623No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22890.20294190400112067No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22520.1996614975144271No Hit
GACTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATT22020.19522851577565212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21950.1946078983322236No Hit
GAATACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAGT21530.19088419367165257No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAGTCTGTC21350.18928832024569356No Hit
GAGTGAAGTCTGTCCCAGATCTGCTGCCACTGAACCTTGATGGGACCCCA20910.18538729631557158No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20840.18476667887214307No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20370.18059967603769453No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19470.17262030890789948No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19000.16845330607345096No Hit
ACAGTATTCCGGGCGGTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGA18690.16570485739541044No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18640.16526155922153293No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18480.16384300506512495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18360.16277908944781894No Hit
GGCCAAAACCGCCCGGAATACTGTAACTCTGTTGACAGTAGTAAGTTGCA18210.16144919492618642No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAGTTATTTAAATTGGTATCAGCAAAAA17900.1587007462481459No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17750.1573708517265134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17740.15728219209173788No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17540.1555089993962279No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT17320.15355848743116687No Hit
ACTTACTACTGTCAACAGAGTTACAGTATTCCGGGCGGTTTTGGCCAGGGGACCAACCTGGAGATCAAGCGAA17010.15081003875312637No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16730.14832756897941235No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16330.14478118358839234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15730.1394616055018623No Hit
GATCTGGGACAGACTTCACTCTTACCATCAGCAGTCTGCAACCTGAAGAT15710.1392842862323113No Hit
GTATTAGTAGTTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCT15630.13857500915410728No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15470.13715645499769927No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTAAGAGTGAAGTCTGTCCCAGATCTGCTGCCA15440.1368904760933728No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15410.1366244971890463No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGGTTGGTCCCCTGGCCAAAACCGCCCGGAATA15370.1362698586499443No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14700.13032966311998573No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14640.12979770531133275No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGGTTGGTCCCCTGGCCAAAACCG14610.12953172640700622No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14360.12731523553761873No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14270.12651729882463922No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14150.1254533832073332No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14050.12456678685957821No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT13940.1235915308770477No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13850.1227935941640682No Hit
GTCCCAGATCTGCTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT13660.12110906110333369No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13470.11942452804259919No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12960.11490288666904866No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12820.11366165178219165No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12510.11091320310415112No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12460.11046990493027364No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12390.10984928748684512No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGCAGATCTGGGACAGA12290.10896269113909011No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT12160.1078101158870086No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11900.1055049653828456No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11890.10541630574807011No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGCAGATCTGGGACAGACTTCACTCTTA11860.10515032684374359No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11570.10257919743525408No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTATTCCGGGCGGTTTTGGCCAGGGGA11480.10178126072227457No Hit
CTAATACTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA11400.10107198364407058No Hit
GAGTATTAGTAGTTATTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC11390.10098332400929509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG200.0077882351.0132181
GTGGTAT3300.043.283941
CGATTAA750.001329376839.5351270
GACCGGA850.002457928634.88392670
GTATCAA26300.033.8794981
GGTACTA450.003967934831.6387560
TATCAAC29400.030.5276852
ATCAACG29750.029.9280323
TCAACGC30050.029.7357434
CAACGCA30050.029.7304695
AACGCAG30250.029.5339056
TCTCGCG5100.029.0699470
TGTTAGA16350.028.56321770
ACGCAGA32100.027.8317957
CGCAGAG32150.027.7885118
AGAGTAC32250.027.59701311
TACTAGC500.00830974827.1973922
GAGTTTA3900.027.0326441
GCAGAGT33050.027.0317889
ATAATAC659.454443E-426.1408983
Produced by FastQC (version 0.11.5)