FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160981
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40990.3530634868270885No Hit
GTAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAAAGGCA37870.326189662018586No Hit
GTTTTAGCCTGGTACCAGCAGAAGCCAGGGAAAGCTCCTAAGTTCCTCAT34660.29854063072522286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33890.2919083085769707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30910.2662403605226959No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT28030.24143375300715517No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27810.23953880382194026No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGTTTTAGCCTGGTACCAGCAGAAG24090.20749693578103345No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT23510.20250116065637594No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23250.2002616752556674No Hit
CCCTTACGGAGCTGTCTCGCACAGTAATAGACAGCCGTGTCCTCAGCTCT22570.19440455959227584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21800.18777223744402363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21650.18648022663592256No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT20900.18002017259541717No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20600.17743615097921497No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19740.17002862234610214No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19610.16890887964574786No Hit
ACTTATTACTGTCAACACTTTGGTAATTACCAAGTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAGACGAA19490.167875270999267No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19420.16727233262215316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19390.16701393046053295No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19330.1664971261372925No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACACTTTGGTAATTAC18800.16193202128200204No Hit
GGCTAAAACACTGCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTC18400.15848665912706583No Hit
GTCTATTACTGTGCGAGACAGCTCCGTAAGGGTGTCTGGTACATTGATTA18140.1562471737263573No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC18110.15598877156473706No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG18030.15529969913374983No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT17940.15452449264888918No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT17620.1517682029249402No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGAGGCATCATAGATGAGGAACTTAGGAGCTTTCCCTGGCTTCTG15440.13299097918053784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15180.1307514937798293No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14690.12653092514003245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14620.1259279867629186No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT14330.12343009920058985No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14310.12325783109284304No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14290.12308556298509622No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCACCGCCGAAAGTG14220.12248262460798238No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGTTTTAGCCTGGTA13960.12024313920727385No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13950.12015700515340044No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13690.11791751975269191No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA13660.11765911759107169No Hit
AGTCAGGGCATTAGCAGTGTTTTAGCCTGGTACCAGCAGAAGCCAGGGAA13650.11757298353719829No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13250.11412762138226207No Hit
CATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG13010.11206040408930035No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12750.1098209186885918No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12600.10852890788049072No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12460.10732303112626305No Hit
GGGCATTAGCAGTGTTTTAGCCTGGTACCAGCAGAAGCCAGGGAAAGCTCCTAAGTTCCTCATCTATGATGCCTC12400.10680622680302261No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12380.1066339586952758No Hit
TCTATGATGCCTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGATTTCACT12310.10603102031816196No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12200.1050835457255545No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGTATAACTAC12150.1046528754561875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12150.1046528754561875No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12140.10456674140231408No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACACTTTGGTAATTACCAAGTCACTTT12080.10404993707907365No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12070.10396380302520024No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11940.10284406032484597No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11900.10249952410935236No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA11890.10241339005547893No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11820.10181045167836511No Hit
CAGTAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAAAGG11780.10146591546287148No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11770.10137978140899809No Hit
GTACCAGCAGAAGCCAGGGAAAGCTCCTAAGTTCCTCATCTATGATGCCT11660.10043230681639062No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11610.1000016365470236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA150.002387896468.76305438
CGTATGT150.002478060868.12331414
GTGGTAT3700.047.882811
CTTATAC5900.045.619771
TCGACGT6050.043.46915429
TGGTATC4250.042.486042
ACGAGAC6350.042.38870222
CTCGACG6300.042.25712228
CGACGTC6250.042.0963230
CCCACGA6400.042.052119
ACTCGAC6350.041.91172427
TATACAC6600.041.2957533
GACGTCA6300.041.24837531
GGTATCA12800.040.724851
CGAGACT6550.040.5829523
GTCGTAT856.730261E-1140.50344539
CACGAGA6650.040.4746821
ATCTCCG6850.040.2738210
CGCGTAT602.9587136E-740.16591639
CGTATGC4050.040.0388141
Produced by FastQC (version 0.11.5)