FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160981
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61920.5333420615841258No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39210.33773162523762235No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26440.2277384384412837No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT26110.22489601466346132No Hit
GTTTTAGCCTGGTACCAGCAGAAGCCAGGGAAAGCTCCTAAGTTCCTCAT25460.21929730116168997No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25010.21542126873738673No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22410.19302641473030135No Hit
GTAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAAAGGCA21630.18630795852817575No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20480.17640254233273414No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20100.17312944828554472No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19070.16425764073658397No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGTTTTAGCCTGGTACCAGCAGAAG19050.16408537262883718No Hit
ACTTATTACTGTCAACACTTTGGTAATTACCAAGTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAGACGAA18650.16064001047390095No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT18560.15986480398904032No Hit
GTCTATTACTGTGCGAGACAGCTCCGTAAGGGTGTCTGGTACATTGATTA18380.15831439101931902No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18230.15702238021121792No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT17910.15426609048726894No Hit
GGCTAAAACACTGCTAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTC17870.15392155427177534No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17640.151940471032687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17610.1516820688710668No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACACTTTGGTAATTAC17470.15047619211683913No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17390.1497871196858519No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17160.14780603644676354No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16780.14453294239957415No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15950.13738381592808152No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC15330.13204350458793038No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15240.13126829810306972No Hit
GTAGTTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG15120.13023468945658886No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15100.13006242134884205No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCACCGCCGAAAGTG14900.12833974027137396No Hit
GAGTGAAATCTGTCCCAGACCCACTGCCGCTGAACCTTGATGGGACCCCA14780.1273061316248931No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG14700.12661705919390584No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCG14250.1227410267696026No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGTATAACTAC14040.1209322116382611No Hit
GTATAACTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCACGCCTCCC13980.12041540731502066No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13720.11817592191431213No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT13690.11791751975269191No Hit
GTCCCAGACCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACT13690.11791751975269191No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13680.11783138569881849No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13610.11722844732170468No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG13510.1163671067829706No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13170.11343854895127482No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13150.11326628084352801No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13110.11292174462803439No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12740.1097347846347184No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGATTTCACT12710.10947638247309818No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12590.10844277382661732No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA12200.1050835457255545No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12120.10439447329456727No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12030.10361926680970662No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGTTTTAGCCTGGTA11940.10284406032484597No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGAGGCATCATAGATGAGGAACTTAGGAGCTTTCCCTGGCTTCTG11940.10284406032484597No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11870.10224112194773213No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11840.10198271978611193No Hit
CCCTTACGGAGCTGTCTCGCACAGTAATAGACAGCCGTGTCCTCAGCTCT11760.10129364735512467No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11680.10060457492413744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.041.9590451
GTGGTAT3150.036.6758581
GTATCAA31350.036.4177971
TCTGGAG11450.035.1797970
GCTTATA400.002797413933.9791031
CGGGTCA808.121788E-833.97324817
TGGTATC3700.032.1271552
GACAGGT2700.031.4621351
ATCAACG36500.031.0664143
TCAACGC36750.030.9394574
GCTTGAC1101.2460077E-930.8900971
CAACGCA37050.030.7792195
TATCAAC37100.030.5758482
AACGCAG37100.030.5547756
ACGCAGA38900.029.2281787
GACCGGA1205.2166247E-428.93739770
CGCAGAG39350.028.893938
ACGGGTC1452.7284841E-1128.1109416
AGAGTAC40250.028.07679211
GCAGAGT40500.027.9058829
Produced by FastQC (version 0.11.5)