FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775154
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG20640.2662696702848724No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT15020.1937679480464527No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC12900.16641854392804525No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT12500.16125827900004386No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG11170.1441003981144392No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT10690.1379080802008375No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC9740.12565245099683417No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT9290.11984715295283259No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC9230.11907311321363238No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC9200.11868609334403227No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC8650.11159072906803036No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA8520.1099136429664299No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCG8350.10772053037202929No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA8080.10423735154562834No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA7900.10191523232802772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.041.3161541
AGGGGGG3700.038.7752270
GTATCAA46800.037.6940161
CTCGTAT1700.036.4916139
TTATACA5300.034.8695032
TATACAC5450.033.909793
TCGTATG1650.033.51751740
CGTATGC1900.032.82416541
CCCACGA5550.031.48453919
GACCGGA2900.031.48207770
CACGAGA5200.031.02279321
GTATGCC2150.030.70062342
GATTACG450.004645166530.63493239
ATCTCCG5850.030.43455110
TCAACGC59150.030.391914
AACGCAG59600.030.2751986
ATCAACG59200.030.2527223
CAACGCA59750.030.1991925
CGAGACT5400.029.88537423
ACGAGAC5400.029.88151622
Produced by FastQC (version 0.11.5)