FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775154
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38810.5006747046393362No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG23660.305229670491283No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT15320.19763814674245375No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC12700.16383841146404457No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT11980.15454993459364205No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC11580.14938966966564063No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT10600.1367470205920372No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG9050.11675099399603177No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC8970.11571894101043148No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT8860.1142998681552311No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT8730.11262278205363063No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC8550.11030066283603002No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA8260.10655947076322898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.038.211081
GTATCAA48350.036.5014231
GTGGTAT7100.031.2487451
TGGTATC7550.031.1672232
GACCGGA3150.029.91958470
TATCCGT450.00526715729.85782411
AACGCAG60300.029.3026686
ATCAACG61550.028.5548883
TCAACGC61850.028.45974
CGTCACT2050.027.8767431
CGCAGAG63400.027.8698868
CAACGCA63300.027.8077775
ACGCAGA63850.027.6208537
GCAGAGT63600.027.5709749
AGAGTAC63500.027.55971311
CAGAGTA64000.027.34616710
GTATGTA908.443694E-626.1458341
TATCAAC70700.025.2477172
ACGGGTA5250.024.96710616
CGCCGTA951.2676652E-524.81138625
Produced by FastQC (version 0.11.5)