FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668230
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT109660.4109840605944765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC87310.3272206668840393No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC69290.25968525951660837No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65000.24360718528762512No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA57090.21396206473954646No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT57030.21373719656851173No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56830.21298763599839596No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56410.21141355880115284No Hit
GAGTTACAGTACACTCAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT54780.2053046401547093No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTACCTATTTAAATTGGTATCAGCAGAAA52020.19496070428711168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49700.18626580167376874No Hit
ACAGTACACTCAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA47750.17895758611514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT44750.16771417756340345No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44190.1656154079670793No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG43850.1643411549978825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42020.1574826757813232No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41830.1567705932397132No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40390.1513737571348797No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG39970.14979967993763657No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC39760.149012641339015No Hit
ACTTACTACTGTCAACAGAGTTACAGTACACTCAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA39580.14833803682591082No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC39450.14785082245533557No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39200.14691387174269085No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG38880.14571457483050562No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC38490.14425293171877987No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT38310.14357832720567568No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37330.1399054804121084No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC36970.13855627138590002No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT36830.13803157898681898No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36780.13784418884429003No Hit
GTGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT36170.13555802910543693No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34160.12802494537577344No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACAGTACACTCAA33560.12577626366542613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTTGAGTGTA33540.12570130760841458No Hit
CCATTCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG33090.12401479632565407No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGAC32810.12296541152749199No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA32660.12240324109990518No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31870.1194424768479479No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31710.11884282839185528No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA31670.11869291627783211No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31390.11764353147967004No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA31270.11719379513760059No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG31100.11655666865300217No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30940.11595702019690957No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30780.11535737174081695No Hit
GTTACAGTACACTCAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA30350.11374581651506804No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30040.11258399763138861No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29700.11130974466219178No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29480.11048522803506446No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG29480.11048522803506446No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG29380.11011044775000656No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT29300.10981062352196025No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCGCGGTAAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG29060.10891115083782132No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT28920.1083864584387403No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG28800.10793672209667082No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28190.10565056235781772No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28080.10523830404425406No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28080.10523830404425406No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACAGTACA27770.10407648516057462No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG27440.10283971021988358No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC27350.10250240796333149No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27200.10194023753574467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6600.040.2811241
GTATCAA56750.036.816761
CGTATGC2400.035.9612241
TCGTATG2450.033.77654340
ATCAACG66300.031.2045543
TATCAAC66850.030.896842
TCAACGC67550.030.6265454
AACGCAG68450.030.2730856
CAACGCA68700.030.1138765
AAACTCG1154.8339917E-429.32007670
AATGGTA11550.029.19314870
ATCTCGT2600.029.06479337
CGAGACA3550.028.8034123
GTGCTAG3700.028.556891
ACGCAGA72800.028.4593857
CGCAGAG73500.028.1403968
AGAGTAC74200.027.87492211
ACATGCG3200.027.72497627
TACGTAC2950.027.72210712
ATACGTA2950.027.72158811
Produced by FastQC (version 0.11.5)