FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053965
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTC80720.3929959858128059No Hit
CACCTGCACCTTGCGCAGTGGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC77690.37824403044842536No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC72970.35526408677849913No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67090.32663652983376057No Hit
GACTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAG57650.2806766424939081No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG54270.2642206658828169No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53690.2613968592454107No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAG52570.2559439912559367No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAAGAGATGCAGAGAGGGAAGACGG52370.2549702648292449No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCA50570.2462067269890188No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG50100.24391846988629312No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG49080.23895246511016496No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA47610.2317955758739803No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC46590.2268295710978522No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA45680.2223991158564046No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGAATTTTACTCATCTCTGGG45390.22098721253770146No Hit
GTAATACACAGCCGTGTCGTCGACTCTCAGGCTGTTCAATTGTAGATACA45270.2204029766816864No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAGTCTCAC44320.2157777761549004No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT43920.21383032330151683No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT43680.21266185158948667No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC43650.21251579262548292No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT43400.21129863459211817No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42150.2052128444252945No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG41990.2044338632839411No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGC41530.20219429250254994No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41440.20175611561053866No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG41240.20078238918384686No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC41100.20010078068516257No Hit
GACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACACCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAG40620.1977638372611023No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG39690.19323600937698548No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC38350.1867120423181505No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA37930.18466721682209775No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37820.18413166728741726No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG35610.173371990272473No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35570.17317724498713466No Hit
CTATTACTGTATGATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCC35480.17273906809512335No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAG35340.1720574595964391No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35260.17166796902576237No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATCTGG35240.1715705963830932No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA34530.16811386756833735No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAG33610.16363472600555512No Hit
CCCAAGAGTTGCTGTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCA33520.1631965491135438No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA33320.162222822686852No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32980.16056748776147597No Hit
ATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGAGACT32710.15925295708544207No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC32620.15881478019343076No Hit
GTATATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGA32050.15603965987735915No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31490.15331322588262214No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGG31180.15180394992124988No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT30960.15073285085188892No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC30810.15000255603187007No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30760.14975912442519712No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA30580.14888277064117453No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28490.13870732948224532No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAACAC28220.13739279880621139No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT28200.13729542616354223No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27930.1359808954875083No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG27920.13593220916617374No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATCTGGCACAGCAACTCTT27300.13291365724342916No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCTTGGAGCATCAGCCAGT26740.13018722324869217No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC26350.12828845671664318No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC25960.1263896901845942No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25790.12556202272190617No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG25500.12415011940320309No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA25450.12390668779653012No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG25280.1230790203338421No Hit
GGCATAACTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGG25250.12293296136983833No Hit
GTCTCACCTGCACCTTGCGCAGTGGCATAACTATTGCTACCTACAGGATA25090.1221539802284849No Hit
GTGTATTACTGTGCGAGAGAGGCAGTGACTGGCCCAGATTACTGGGGCCA24800.12074207690978181No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC24430.11894068302040199No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC24060.11713928913102219No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24030.11699323016701843No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23890.11631162166833417No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT23770.11572738581231909No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC23370.11377993295893553No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23330.11358518767359715No Hit
GTGCCAGATCATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGC23170.11280620653224373No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG22800.11100481264286392No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22710.11056663575085263No Hit
GCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC22680.11042057678684884No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC22520.10964159564549542No Hit
ATCTGGCACAGCAACTCTTGGGTGTTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22450.1093007913961533No Hit
AGTATATCCTGTAGGTAGCAATAGTTATGCCACTGCGCAAGGTGCAGGTGAGACTGGCTGATGCTCCAAGAGATG22430.10920341875348412No Hit
ATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAA22340.10876524186147281No Hit
GCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATTCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG22070.10745071118543889No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG21940.10681778900808922No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT21480.1045782182266981No Hit
CTATTGCTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT21460.10448084558402895No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21440.10438347294135977No Hit
TCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGCCCTGCTGCTTATC21420.10428610029869058No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21260.10350711915733715No Hit
CTCTTGGAGCATCAGCCAGTCTCACCTGCACCTTGCGCAGTGGCATAACT21100.10272813801598371No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC20930.1019004705532957No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20660.10058593987726178No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20550.10005039034258129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)