FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233021
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT51370.4166190194651997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43470.35254874004578995No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42740.3466283218209585No Hit
GTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGA35280.2861265136603513No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28420.23049080267083855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28050.22749004274866366No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27870.22603021359733533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26340.21362166581104458No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25790.2091610767375414No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24580.19934778077583432No Hit
GTACTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC24430.1981312564830607No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23660.1918864317801562No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23650.19180533016063797No Hit
AACTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT21310.17282755119336976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20940.1698267912711949No Hit
GATTTCACCCTCACCATCAATAGCCTGGAACCTGAAGATGCTGCAGCGTATTACTGTCATCAGAGTAGTACTTTA20900.16950238479312194No Hit
CAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA19900.16139222284129792No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19890.16131112122177968No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19830.16082451150467023No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19800.16058120664611553No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18910.15336316250899212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17400.1411168179617379No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16570.13438538354172394No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16280.13203343657569497No Hit
GCGTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15840.12846496531689242No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAC15570.12627522158989993No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15450.12530200215568105No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15430.12513979891664456No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15330.12432878272146217No Hit
GTGTAAGTTACTACCAATGCTCTGACTGGCCCGGCACGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA15320.12424768110194392No Hit
GTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14870.12059810822362312No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14670.11897607583325831No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14500.11759734830144822No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14360.11646192562819287No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14290.11589421429156518No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.11483989323782806No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT14030.11378557218409094No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13830.11216353979372615No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13630.11054150740336134No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13580.11013599930577013No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGATTCAGGGAGA13530.10973049120817893No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT13480.10932498311058773No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13420.10883837339347831No Hit
GTCCATCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGC13060.10591871509082165No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12790.10372897136382918No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA12730.10324236164671971No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12590.10210693897346436No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG12570.10194473573442789No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12520.10153922763683668No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12520.10153922763683668No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12470.10113371953924548No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12460.10105261791972724No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12440.10089041468069075No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12410.10064710982213604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAC403.5293488E-545.74910466
GTGGTAT4650.043.977571
GGTATCA15950.042.9505041
TGGTATC5000.040.2158552
GTATCAA39600.038.2999081
TCGTATG3250.037.17183340
CGTATGC3300.036.6477741
CTTATAC4100.036.5764661
TATACAC4500.035.5959553
TAGGGAT6700.034.70208470
GTACGTA500.002585423434.52911469
AGAACCG502.0004487E-434.0798535
ATCTCGT3550.033.89824737
TATCAAC45450.033.1439062
ACGAGAC4700.032.63361722
ATGCCGT3950.032.5030344
ATCAACG46750.032.440963
CCGTCTT4200.032.33149747
TTATACA4750.032.2874872
CACGAGA4450.032.16655721
Produced by FastQC (version 0.11.5)