FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233021
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT47000.3811776117357287No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40990.3324355384052664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38380.3112680157110057No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31400.2546590852872741No Hit
GTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGA31190.252955951277391No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30510.24744104115015073No Hit
GTACTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC27750.22505699416311645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26900.218163356504066No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23680.1920486350191927No Hit
CAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA22310.1809377131451938No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21800.17680153054976355No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21370.17331416091047924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21100.17112441718348673No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20320.164798490861064No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19820.16074340988515198No Hit
GATTTCACCCTCACCATCAATAGCCTGGAACCTGAAGATGCTGCAGCGTATTACTGTCATCAGAGTAGTACTTTA19530.158391462919123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19410.15741824348490416No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19090.15482299166032049No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18460.14971358963067136No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17980.1458207118937958No Hit
AACTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT17480.1417656309178838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16520.13397987544413276No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16430.13324996086846858No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.1328444527708774No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15880.12878937179496538No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15870.12870827017544714No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15030.12189573413591497No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15020.12181463251639672No Hit
GCGTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14760.11970599040892248No Hit
GTGTAAGTTACTACCAATGCTCTGACTGGCCCGGCACGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA14260.11565090943301047No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT14210.11524540133541927No Hit
GTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14180.11500209647686455No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13680.11094701550095254No Hit
GGGTGAAATCTGTCCCCGATCCACTGCCACTGAACCTCGAGGGGACCCCT13650.11070371064239781No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13270.1076218491007047No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13040.10575651185178517No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12900.1046210891785298No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAC12770.1035667681247927No Hit
GTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA12660.10267465031009203No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12540.10170143087587316No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12450.100971516300209No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGATTCAGGGAGA12430.10080931306117252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.048.5083471
TGGTATC5050.041.762382
GGTATCA16050.041.137771
GTATCAA39100.037.1676561
TATCAAC46000.031.4279462
TAGGGAT6350.030.51103670
ATCAACG47250.030.3719163
TCAACGC47400.030.199164
AACGCAG48250.029.808096
CAACGCA48350.029.7464395
GACAGGT3450.028.6083621
ACGCAGA51050.028.1731727
CGCAGAG52100.027.8664258
AGAGTAC52950.027.2873411
GCAGAGT53350.027.2761349
TAACGGC1503.0378942E-822.89012136
GACGTGT5000.022.88367862
AATGGTA4850.022.82710870
GAGTACG47850.022.59532712
AGTACGG48050.022.3633913
Produced by FastQC (version 0.11.5)