FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456694
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATTGTCAGCACTATGGTGATTCACCTCCGACATACACTTTTGGCC68140.4677715429596058No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38750.2660133150819596No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35160.241368468600818No Hit
CAATAATAGAGTGCAAAATCTTCAGGCTCTACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG34450.2364944181825421No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27140.18631229345353245No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC25960.17821175895555277No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24780.1701112244575731No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24710.16963068427548958No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23640.16228528434935546No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23160.15899015167221117No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22450.1541161012539353No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22040.15130150875887455No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21740.14924205083565936No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21060.144573946209705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20810.14285773127369233No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20660.14182800231208476No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19870.1364047631142848No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19750.13558097994499874No Hit
GTCAGAGTGTGACCACCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATGTCTGGTG19710.13530638555523672No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18810.1291280117855912No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18720.12851017440862667No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18160.12466585295195834No Hit
CCATAGTGCTGACAATAATAGAGTGCAAAATCTTCAGGCTCTACTCTGGT18150.12459720435451782No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17990.12349882679546975No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGACCACCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCT17700.12150801746969508No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGAGTAGAGCCTGAAGATT17630.12102747728761154No Hit
GAGTGTGACCACCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATGTCTGGTGCATC17410.11951720814392042No Hit
CCCTAAGACTCTCCTGTGCAACCTCTGGATTCAACTTTGCCAGTTATGCC17310.11883072216951536No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGGTGGCTTCTT17240.11835018198743182No Hit
CCTCATGTCTGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGCGGCAGTGGGTCTGGGACAGACTT16990.11663396705141917No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16810.11539829229749007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16550.11361342876403692No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCAGA16410.11265234839986984No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16320.11203451102290529No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16010.10990640450224962No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15830.10867072974832051No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCC15730.10798424377391545No Hit
GAGTAGAGCCTGAAGATTTTGCACTCTATTATTGTCAGCACTATGGTGAT15620.10722910920206989No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT15540.10667992042254584No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15460.10613073164302181No Hit
GGACTGGAGTGGGTCTCGAGCGTTAGTGGCAGTGGTGGCCTGACATACTA15420.10585613725325978No Hit
CCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATGTCTGGTGCATCCACCAGGGCCA15360.10544424566861675No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15340.10530694847373573No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15270.10482640829165218No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15140.10393397652492561No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15130.10386532792748511No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14970.10276695036843701No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCAGAGTGTGACCAC14890.10221776158891298No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCAGAGTGTGACCACCT14730.1011193840298649No Hit
GTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTAAGACTCTCCTGTG14620.10036424945801933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)