FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456694
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64550.44312669647846425No Hit
CTATTATTGTCAGCACTATGGTGATTCACCTCCGACATACACTTTTGGCC59510.4085278033684494No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38360.26333601978177984No Hit
CAATAATAGAGTGCAAAATCTTCAGGCTCTACTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG33860.23244415093355225No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26660.1830171607763882No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25550.17539716646049203No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24710.16963068427548958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22100.15171340034351757No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC21370.14670205273036066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21360.14663340413292017No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19480.1337274678141051No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18860.12947125477279373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18470.12679395947261402No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18460.12672531087517352No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18440.1265880136802925No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18350.12597017630332794No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17330.11896801936439637No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGAGTAGAGCCTGAAGATT17300.11876207357207486No Hit
GTCCCAGACCCACTGCCGCTGAACCTGTCTGGGATGCCAGTGGCCCTGGT17190.11800693900022928No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16990.11663396705141917No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16770.11512369790772806No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16670.114437211933323No Hit
CCCTAAGACTCTCCTGTGCAACCTCTGGATTCAACTTTGCCAGTTATGCC16410.11265234839986984No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16330.11210315962034578No Hit
GTCAGAGTGTGACCACCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATGTCTGGTG16110.11059289047665467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16040.11011235029457114No Hit
CCATAGTGCTGACAATAATAGAGTGCAAAATCTTCAGGCTCTACTCTGGT15970.10963181011248758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15590.10702316340974838No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15530.10661127182510534No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACTCTCTCCTGCAGGGCCAGTCAGA15380.10558154286349776No Hit
GAGTGTGACCACCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATGTCTGGTGCATC15330.10523829987629522No Hit
GAGTAGAGCCTGAAGATTTTGCACTCTATTATTGTCAGCACTATGGTGAT15200.10434586810956865No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15100.1036593821351636No Hit
GGACTGAACAGAGAGAACTCACCATGGAATTTGGGCTGAGGTGGCTTCTT14960.10269830177099652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14860.10201181579659147No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGACCACCTCCTTAACTTGGTATCAACAAAAACCTGGCCAGGCTCCCAGGCT14860.10201181579659147No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14670.10070749244522186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)