FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371448
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTACCTCACGGTGGACGTTCGGCCAAGGGA49060.3577240989085988No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36610.2669441349580881No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32970.24040284429303918No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31880.23245504022026356No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT30810.22465306741487828No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30280.22078853882903327No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26430.19271601985638537No Hit
GTACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC26080.19016397267705376No Hit
CTATGGTACCTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT25730.1876119254977221No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25400.18520570958578086No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24970.1820703373368877No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24180.17631000227496776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23970.1747787739673688No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23320.17003925777718149No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACCCTGTATCTG22310.16267477877396735No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA22020.1605602253968069No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21400.15603945610770514No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCAACTATGCCATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGC21170.1543623965327158No Hit
GTGTATTACTGTCAGCACTATGGTACCTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21020.15326866202728795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20720.15108119301643227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20530.14969579597622365No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCACCAACTACTTAGCCTGGTACC19880.14495627978603637No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19760.1440812921816941No Hit
GTATGTGCTAGTACCACGACCACTAACAGCTGAGACCCACTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCC19060.13897719782303083No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGGG19030.13875845092194528No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18990.13846678838716453No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18440.13445642853392908No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT18220.13285228459263493No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17970.1310293937502552No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17870.13030023741330332No Hit
ATATTACTGTGCGAAAGGGTGGGGCTATGGTGACTACTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGT17800.129789827977437No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17710.12913358727418028No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17390.12680028699593424No Hit
CCATAGCCCCACCCTTTCGCACAGTAATATAAGGCCGTGTCCTCGGCTCT17260.12585238375789676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17170.12519614305464005No Hit
GTCTCCAGGCACCCTGTCTTTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAC16950.1235919991133459No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16840.1227899271426988No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16730.12198785517205174No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16700.12176910827096615No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16370.11936289235902492No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16240.11841498912098745No Hit
GCCTTATATTACTGTGCGAAAGGGTGGGGCTATGGTGACTACTACTTTGA16070.11717542334816924No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16010.1167379295459981No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCCCATGGCATAGTTGG15930.1161546044764366No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15710.11455046053514242No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15640.11404005109927609No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT15510.11309214786123863No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15410.11236299152428673No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15390.11221716025689636No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15210.11090467885038295No Hit
CTCCAGGCACCCTGTCTTTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGC15150.1104671850482118No Hit
ATGGTACCTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15020.10951928181017435No Hit
GTCTTTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14810.10798805350257538No Hit
GTGTTACCACCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT14780.10776930660148983No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14770.10769639096779463No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGG14610.10652974082867159No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14570.10623807829389083No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14490.10565475322432932No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14430.10521725942215819No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14260.10397769364933997No Hit
CATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14240.1038318623819496No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14160.10324853731238809No Hit
GTACTAGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCC14130.1030297904113025No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTAGTCAC14120.1029568747776073No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14070.10259229660913138No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13910.10142564647000833No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCA13810.10069649013305644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2000.043.13030241
TCGTATG2000.043.08940
GGTATCA11200.041.7596321
ACGAGAC2500.040.9536722
TCTCCGA2450.040.3920611
ATGCCGT2150.040.25774844
GTATCAA28800.038.880831
CGAGACG2600.038.067323
TATACAC2800.037.7956623
GCCGTCT2300.037.72434246
ATCTCGT2150.036.78404237
CACGAGA2750.035.98958621
ATCTCCG2850.035.9203410
GCTACAT2600.035.55866232
GGAGCTA2600.035.48855629
TACATCT2350.035.0201934
TATCAAC32000.034.991472
AACTGTG25600.033.59803870
CTTATAC2950.033.5606271
CGTCTTC2700.033.51791448
Produced by FastQC (version 0.11.5)