FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371448
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTACCTCACGGTGGACGTTCGGCCAAGGGA44960.32782868909357116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35640.2598713184896547No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29320.21378863799429507No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28390.20700748406064245No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA27750.20234088350415033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26840.19570556083788812No Hit
GTACCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC24330.17740373678039562No Hit
CTATGGTACCTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23810.17361212382824578No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23310.1699663421434863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23290.16982051087609593No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22150.16150812863484434No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21770.15873733455442712No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCAACTATGCCATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGC21050.15348740892837354No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20740.15122702428382265No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20730.15115410865012746No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20650.15057078358056594No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20610.1502791210457852No Hit
GTGTATTACTGTCAGCACTATGGTACCTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20260.14772707386645356No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA19430.14167507626975284No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCCAGAACACCCTGTATCTG19240.14028967922954425No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCACCAACTACTTAGCCTGGTACC19080.1391230290904212No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18420.13431059726653874No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18160.1324147907904638No Hit
GTATGTGCTAGTACCACGACCACTAACAGCTGAGACCCACTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCC18010.13132105628503596No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT17620.12847734657092358No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGGG17120.1248315648861641No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17100.12468573361877372No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16710.12184202390466134No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16520.12045662686445277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16000.11666501391230291No Hit
ATATTACTGTGCGAAAGGGTGGGGCTATGGTGACTACTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGT15980.11651918264491254No Hit
GTCTCCAGGCACCCTGTCTTTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAC15780.11506086997100874No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15770.11498795433731354No Hit
GCCTTATATTACTGTGCGAAAGGGTGGGGCTATGGTGACTACTACTTTGA15180.11068593194929739No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15080.1099567756123455No Hit
CTCCAGGCACCCTGTCTTTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGC14940.10893595674061284No Hit
GTCTTTGTCTTCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14840.10820680040366094No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCCCATGGCATAGTTGG14730.10740472843301387No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14730.10740472843301387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14710.10725889716562348No Hit
CCATAGCCCCACCCTTTCGCACAGTAATATAAGGCCGTGTCCTCGGCTCT14560.10616516266019566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14490.10565475322432932No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGG14460.10543600632324376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14260.10397769364933997No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14210.10361311548086401No Hit
GTGTGAGGTGCAGCTGTTGGAGTTTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC14060.10251938097543618No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13970.10186314027217948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13880.10120689956892277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13840.10091523703414203No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13790.10055065886566607No Hit
GTGTTACCACCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT13770.10040482759827568No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13750.10025899633088532No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13740.10018608069719012No Hit
ATGGTACCTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA13730.10011316506349494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)