FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998311
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC98180.4913149154460943No Hit
GCTTAGTAGTTACCCTTCGATCACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCAT89020.44547620465483106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC83400.4173524541475276No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81270.4066934526207382No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC73150.3660591369411468No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA65390.32722634264636485No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG63810.31931966545747886No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC62500.31276412930719993No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60480.3026555926479912No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG60170.3011042825666275No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG53960.2700280386786641No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG53740.2689271089435028No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53060.2655242352166404No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA52390.26217140375046727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG51210.2562664169891473No Hit
GTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACACAGTCACCATCACTT49970.2500611766636925No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT48540.24290513338514375No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT48280.2416040346072258No Hit
CCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCA47420.23730040018795873No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47200.23619947045279738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46870.23454807585005535No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC44690.22363886301982025No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT44200.22118679224605178No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44190.22113674998536265No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG44010.2202359892929579No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCC43150.21593235487369083No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAGTAGTTAC42880.21458121383508372No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA41050.20542348012896888No Hit
GTAGTTACCCTTCGATCACCTTCGGCCAAGGGACACGAGTGGAGATTAAA41030.2053233956075906No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG40870.2045227194365642No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT40830.2043225503938076No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCCCTTGGCCGAAGGTG40790.20412238135105099No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40380.20207064866279573No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT39550.19791714102559613No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG39200.19616566190147577No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39000.19516481668769275No Hit
GGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC37490.1876084353236308No Hit
ATCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG37320.18675771689191523No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG36740.18385526577194441No Hit
ACTTATTACTGTCAACAGCTTAGTAGTTACCCTTCGATCACCTTCGGCCA36500.18265425151540476No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG34940.17484765884789705No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAA34650.17339643328791163No Hit
GCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTGCTA34650.17339643328791163No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34400.17214537677068284No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC33600.16814199591555068No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33340.16684089713763273No Hit
CATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG33190.16609026322729545No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATT32400.16213692463285245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32210.16118612167975854No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA32180.1610359948976911No Hit
CTCGTGTCCCTTGGCCGAAGGTGATCGAAGGGTAACTACTAAGCTGTTGA32160.1609359103763128No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31630.15828367055978773No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC31130.15578155752533013No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA30880.15453050100810134No Hit
GTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTGCTAATG30870.1544804587474122No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT30500.15262889510191358No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC30470.1524787683198461No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAGTAGTTACCCTTCGATCAC29940.14982652850332104No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29680.14852542972540309No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA29590.14807504937920074No Hit
GGCCGAAGGTGATCGAAGGGTAACTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG29550.14787488033644414No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC29450.1473744577295526No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAATCTTG29350.1468740351226611No Hit
GGGTAACTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG29270.14647369703714788No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT29260.14642365477645872No Hit
GGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC28850.14437192208820349No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT28760.14392154174200114No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC28490.14257040070339402No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28300.14161959775030014No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA28130.14076887931858453No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC28070.14046862575444963No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC26990.1350640616000212No Hit
GTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTG26880.13451359673244054No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA26690.13356279377934666No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAATTGCTAATGCC26650.13336262473659005No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT26460.13241182178349617No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT26250.13136093430902396No Hit
GTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT26090.13056025813799754No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT25960.12990970874903857No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25420.12720742667182436No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25140.1258062433725281No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT24950.12485544041943421No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGCCTGCATCTGTAGGAGACACAGTCACCATCA24670.12345425712013795No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24580.1230038767739356No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24260.12140252443188272No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24060.12040167921809969No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCA24050.12035163695741054No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23340.11679863644848074No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23240.11629821384158921No Hit
GGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGG23180.11599796027745432No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG23130.11574774897400855No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC23030.11524732636711704No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22980.11499711506367127No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22960.11489703054229296No Hit
CACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC22860.11439660793540143No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG22760.11389618532850992No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC22720.11369601628575332No Hit
GTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCACTTTGCAAAGT22690.11354588950368587No Hit
GGTAACTACTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT22140.11079356516578251No Hit
CATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGGCATT22070.11044326934095844No Hit
GTGATGGTGACTGTGTCTCCTACAGATGCAGGCAGGGAGGATGGAGACTGGGTCAACTGGATGGCACATCTGGCA22020.11019305803751267No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21980.10999288899475608No Hit
GCCACAGTTCGTTTAATCTCCACTCGTGTCCCTTGGCCGAAGGTGATCGA21520.10769094500305508No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTC21420.10719052239616357No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC21410.1071404801354744No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC21310.10664005752858288No Hit
TGCCCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTACAGATGCA21140.1057893390968673No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT20980.10498866292584087No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20620.1031871415410314No Hit
CCATCCTCCCTGCCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG20220.10118545111346533No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT20060.10038477494243889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG252.7641444E-454.3822
GTGGTAT2450.048.5590021
GTATCAA23850.038.7657621
GACAGGT3450.036.454441
GTGTATA601.4891275E-533.9836513
ATCAACG29000.031.5274983
TGTATAA652.5740497E-531.37030614
TCAACGC29350.030.919924
CAACGCA29550.030.825675
TATCAAC30100.030.4882492
AACGCAG30250.030.2247076
GCTCTAA803.1039053E-629.7401564
AAAACCG2102.2220775E-829.71623670
ACGCAGA30900.029.5844697
AGAGTAC31300.029.20639411
CGCAGAG31350.029.1598138
CAGAGTA32000.028.67370410
GACAGGC3950.028.3977991
GCAGAGT32650.027.9987779
AATGGTA5300.027.47350170
Produced by FastQC (version 0.11.5)