FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470539
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG53040.36068407570285455No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48260.32817898743249924No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45760.31117841825344317No Hit
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGAACTCAGG43750.297509960633482No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39340.26752095660162706No Hit
GATTATTACTGCGCAACATGGGATAACAGCCTGAGTTCTGTGGTATTCGG39150.26622891334401877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC38150.2594286856723963No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT38090.25902067201209894No Hit
ACATGGGATAACAGCCTGAGTTCTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGG33110.22515553820741918No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32230.21917133785639142No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30830.20965101911612No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAC30510.20747494626120083No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30260.20577488934329521No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29780.20251078006091644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29140.19815863435107806No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28840.19611856604959135No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27240.18523820177499545No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27130.18449017673111695No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCGGCTCCAACATTGGAAA27120.18442217445440073No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26220.17830196954994054No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26100.17748594222934583No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25860.17585388758815645No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25180.1712297327714532No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24950.16966568040698002No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23820.16198142313804667No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG23520.15994135483655994No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23510.15987335255984372No Hit
GGATAACAGCCTGAGTTCTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC23090.15701725693776228No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG22910.15579321595687023No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22870.15552120685000534No Hit
GTTTGGGGGCTGTTCCTGGGACTTGCCGGTACCAGGCTACATAATCATTT22000.1496050087756938No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21990.14953700649897758No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAA21970.14940100194554515No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21810.14831296551808554No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG21520.14634089949331505No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21080.14334879931780117No Hit
GTTCTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCC20390.13865664222438165No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20120.13682058075304362No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19920.13546053521871912No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGTC19390.13185641455275923No Hit
GTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCT19150.13022435991156986No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTC19090.1298163462512725No Hit
TGATTATGTAGCCTGGTACCGGCAAGTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACC19020.12934033031425893No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18900.12852430299366424No Hit
GCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGACTCCAAGT18740.12743626656620463No Hit
CCACAGAACTCAGGCTGTTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC18100.12308412085636627No Hit
ACATAATCATTTCCAATGTTGGAGCCGCTTCCAGAGCAGGAGATGGTGACCTTCTGTCCTGGGGCCGCAGACA18020.12254010264263647No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAAC17940.12199608442890668No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17900.12172407532204177No Hit
CTCCTGCTCTGGAAGCGGCTCCAACATTGGAAATGATTATGTAGCCTGGTACCGGCAAGTCCCAGGAACAGCCCC17560.11941199791369016No Hit
CTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTG17540.11927599336025771No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17230.11716792278205475No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17030.11580787724773026No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT16710.11363180439281108No Hit
GCCTGGTACCGGCAAGTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACCCTCAGGGATT16680.11342779756266239No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16630.11308778617908127No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16510.11227175885848659No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16460.11193174747490546No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16250.11050369966386475No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC15860.10785161087193199No Hit
CTGTTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG15820.1075796017650671No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15810.10751159948835087No Hit
GGGTGGCTGACGTGCCAGACTTGGAGTCAGAGAATCGGTCAGGAATCCCT15750.10710358582805353No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15680.10662756989103996No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15610.1061515539540264No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15360.10445149703612076No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA15340.10431549248268832No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15200.10336346060866117No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT15070.10247943101135026No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14950.10166340369075558No Hit
CAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAACAGC14910.10139139458389067No Hit
GTTCCTGGGACTTGCCGGTACCAGGCTACATAATCATTTCCAATGTTGGA14820.10077937409344465No Hit
GTACCAGGCTACATAATCATTTCCAATGTTGGAGCCGCTTCCAGAGCAGGAGATGGTGACCTTCTGTCCTGGGGC14760.10037136043314729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.004596449458.28516470
GTATCGT309.7015545E-656.9108920
GGTATCA12200.043.9456561
AGGGGGG3600.041.5551670
TGGTATC3800.041.336682
TCGTATG2000.039.6937640
GTATCAA32700.036.9684871
CGAGACG3250.036.7769423
TATACAC3600.036.0460133
CTTATAC3650.035.552231
ACGTAGA3500.035.14964327
CGTATGC2400.034.556741
GACGTAG3600.034.162826
TCGCTAT501.9758189E-434.1523424
ATGCCGT2450.033.9683844
AGACGTA3650.033.6879425
CACGAGA3700.033.22478521
TATCAAC36650.032.703522
CGTAGAG3800.032.38569328
ACGAGAC3700.032.30187222
Produced by FastQC (version 0.11.5)