FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470539
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44460.30233812228033397No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43620.2966259310361711No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG39980.2718731023114654No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36710.24963635782526No Hit
GATTATTACTGCGCAACATGGGATAACAGCCTGAGTTCTGTGGTATTCGG34400.2339278319038121No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33480.22767162244591949No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG32420.22046338111399968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32380.2201913720071348No Hit
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGAACTCAGG32040.21787929459878316No Hit
ACATGGGATAACAGCCTGAGTTCTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGG30370.2065229143871737No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30140.20495886202270053No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29990.20393882787195713No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC29840.20291879372121377No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC29580.20115073452659196No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAC28640.19475852051526685No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28190.19169841806303675No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28050.1907463861890096No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27510.18707426324633347No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27320.1857822199887252No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27090.18421816762425205No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25670.1745618443305482No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23740.16143740492431685No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23620.16062137760372217No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCGGCTCCAACATTGGAAA23550.1601453616667086No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG23290.15837730247208676No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22820.15518119546642423No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22480.1528691180580726No Hit
GGATAACAGCCTGAGTTCTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC22470.15280111578135636No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22260.15137306797031563No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22060.15001302243599116No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21140.1437568129780985No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAA20860.14185274923004423No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20680.1406287082491522No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19890.13525652838857044No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG19820.13478051245155687No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19770.13444050106797575No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19560.13301245325693503No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19540.1328764487035026No Hit
GTTCTGTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGTCAGCCC19310.1313123963390294No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19170.13036036446500227No Hit
GTTTGGGGGCTGTTCCTGGGACTTGCCGGTACCAGGCTACATAATCATTT18900.12852430299366424No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18410.12519219143456922No Hit
GTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCT18230.12396815045367718No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG17970.12220009125905534No Hit
GGGTGGCTGACGTGCCAGACTTGGAGTCAGAGAATCGGTCAGGAATCCCT17950.1220640867056229No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC17930.12192808215219046No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT17670.12016002295756861No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17560.11941199791369016No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGTC17180.11682791139847364No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT17140.11655590229160873No Hit
CTCCTGCTCTGGAAGCGGCTCCAACATTGGAAATGATTATGTAGCCTGGTACCGGCAAGTCCCAGGAACAGCCCC17110.11635189546146006No Hit
GCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGACTCCAAGT17090.1162158909080276No Hit
TGATTATGTAGCCTGGTACCGGCAAGTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAAGCGACC17080.11614788863131137No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16730.11376780894624351No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16120.10961967006655382No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT16010.10887164502267536No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15870.10791961314864822No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15820.1075796017650671No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15760.10717158810476975No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA15740.1070355835513373No Hit
CTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTG15540.10567553801701282No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC15500.10540352891014791No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTC15340.10431549248268832No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAAC15280.10390747882239099No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15260.10377147426895852No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14850.10098338092359331No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG14840.10091537864687711No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14740.10023535587971487No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14710.10003134904956618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)