FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119903
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA49720.4439670221438821No Hit
GTCTCTGGTGGCTCCATCAGCAGGAGTAGTTACTTCTGGGACTGGATCCG31470.2810064800255022No Hit
GTATAATAACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30890.2758274600568085No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28110.2510038815861731No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27930.24939659952692333No Hit
GTGTATTACTGTGCGAGACACTACGGTGACTACGACCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT26360.23537752823235583No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT24080.21501862214852535No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24030.21457215490984488No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23970.2140363942234283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22380.1998387360333886No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA20860.1862661319775016No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20720.18501602370919623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18740.1673359210574487No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGGAGTAGTTACTTCTGGG18740.1673359210574487No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18230.1627819552229077No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC16740.14947723151022901No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16340.14590549360078506No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA16270.14528043946663238No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACT16020.14304810327322992No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15690.14010141949793867No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG15630.13956565881152208No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15620.139476365363786No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15240.1360832143498142No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT15160.13536886676792542No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15040.13429734539509225No Hit
CTATAATAGTGAGAGGACCTACTACAACTCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA14950.13349370436546737No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14910.13313653057452296No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14790.1320650092016898No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14650.1308149009333844No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14620.13054702059017612No Hit
CTATTATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGTCCCAGAAGTAACTACTC14410.12867185818771804No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA14370.12831468439677363No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14160.1264395219943156No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14090.1258144678601629No Hit
GTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGGCCTC13870.12385001200996872No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC13770.12295707753260773No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13630.12170696926430236No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13290.11867099204127501No Hit
ATTATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGTCCCAGAAGTAACTACTC12910.11527784102730325No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGCCAGTTATTATACTG12820.11447419999767838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12760.11393843931126178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12550.1120632769088037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12470.11134892932691492No Hit
ATCTATAATAGTGAGAGGACCTACTACAACTCGTCCCTCAAGAGTCGAGT12410.11081316864049832No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12350.11027740795408175No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12300.10983094071540125No Hit
GTATTACTGTGCGAGACACTACGGTGACTACGACCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC12250.10938447347672074No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGGGGT12240.10929518002898465No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12010.10724143073105438No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12000.1071521372833183No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGTGGACGTTCGGCCA11880.10608061591048511No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11700.10447333385123533No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11660.10411616006029092No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG11640.10393757316481873No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11610.10366969282161044No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC11530.10295534523972166No Hit
GTAATAAACTGCAAAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA11320.10108018283726358No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11310.10099088938952748No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11240.10036583525537479No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA11220.1001872483599026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAT501.9397965E-458.41469270
CACATCG200.006660080553.07238450
CGTAAGC200.00734724551.770841
CGTCTTA305.736784E-446.89944548
CGTCGTG305.736784E-446.89944548
TACGGAT13400.046.8625370
TATTCCG451.7902566E-646.26069643
CCGTCGT404.5298428E-543.8553347
GGTATCA9400.043.2051281
CTTATAC8050.042.111751
CGTGTAA350.001070733241.3173157
CGTATGC5450.041.1633341
TGAGCGA8300.041.10770431
TTAACGA350.001141448240.7836553
TCGTATG4900.040.7446240
TATACAC8500.040.6702353
CTCTTAT2250.040.277181
CACGAGA8350.040.2093621
GAGCGAT7750.040.17423632
ACGAGAC8550.040.0631122
Produced by FastQC (version 0.11.5)