FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116561
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC87390.4128867535591934No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCC83360.39384643296366134No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT69840.32996922838510206No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCTAAATTGGTG69050.3262367585909407No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG66130.31244079428847077No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG64500.30473962243469477No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG59500.2811163958893696No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC55840.2638241940581916No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC54440.25720969062550053No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC50040.23642125126561436No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT49460.23368095698635666No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAACTCATAT49280.23283052083072492No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT46000.21733368421699165No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGGAAAGC43340.20476612769487862No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG43100.203632212820703No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42960.2029707624774339No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG41100.19418292220257297No Hit
GATTATTACTGCAACTCATATGCAGGCAACACCAATTTAGGGGTATTCGG40420.19097016339240871No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC40360.19068668467386482No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG39940.1887023336440575No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC39590.18704870778588475No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA39350.18591479291170915No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTATCAACAACA37540.1773631849023014No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37420.17679622746521362No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37290.17618202357503515No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36860.1741504260921372No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36440.1721660750623299No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG36090.17051244920415712No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCTAAATTGGTGT35110.1658822968012734No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34760.16422867094310062No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG34410.16257504508492787No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32940.15562981648060226No Hit
GGATGAGGCTGATTATTACTGCAACTCATATGCAGGCAACACCAATTTAGGGGTATTCGGCGGAGGGACCAAG32260.15241705767043803No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTCCCTGGGTGTTGTTGATACCAGGAGA32220.15222807185807544No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31410.14840110915773275No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30510.14414892837957424No Hit
ATTTAGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC30020.14183385217813235No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT29920.14136138764722583No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG29630.13999124050759698No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC29570.1397077617890531No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28850.13630601716652627No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT28810.13611703135416367No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGGAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG28450.13441615904290025No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27990.13224282220073033No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC27980.13219557574763968No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT27320.12907730984365676No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26900.12709295881384947No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26830.1267622336422149No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG26550.12543933295567666No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG26290.12421092517531979No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT26210.12383295355059458No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT25880.1222738205986031No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG25850.12213208123933116No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC25560.1207619340997023No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25530.12062019474043036No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25030.11825787208589783No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC24720.11679323204008767No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24680.11660424622772507No Hit
CCCCTAAATTGGTGTTGCCTGCATATGAGTTGCAGTAATAATCAGCCTCA24240.11452540229173645No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC24190.1142891700262832No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC24140.11405293776082996No Hit
GCATATGAGTTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA23850.1126827906212011No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23440.11074568604448443No Hit
CTCATATGCAGGCAACACCAATTTAGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22720.1073439414219576No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA22640.1069659697972324No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22410.10587930137614743No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22350.10559582265760353No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT22320.10545408329833159No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC22250.10512335812669703No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22100.10441466133033729No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22030.10408393615870272No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC21960.10375321098706816No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG21700.10252480320671127No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA21530.10172161350417021No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA21520.10167436705107956No Hit
GCTTGGTCCCTCCGCCGAATACCCCTAAATTGGTGTTGCCTGCATATGAGTTGCAGTAATAATCAGCCTCATCCT21440.10129639542635435No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC21380.10101291670781046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5850.048.4247361
TCTCGTA2150.035.1813338
TTCGCAT400.002446167434.9229850
GCTAATC2350.032.0597233
ATCAACG55400.031.3568573
TCAACGC56250.030.883024
TCGTATG2800.030.76831240
CAACGCA56850.030.6171115
AACGCAG57450.030.2372286
CGTATGC2900.029.74637241
TCGCTAA2850.028.8028931
CCGTTAA953.5328048E-728.73212616
ACGCAGA61200.028.376417
CGCAGAG61350.028.3063628
ATCTCGT2550.028.29081537
AGGGGGG3500.028.14871270
ACGAGAC3400.028.09965522
AGAGTAC62500.027.73093611
GCAGAGT63200.027.5857419
CAGAGTA63200.027.53175710
Produced by FastQC (version 0.11.5)