FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825228
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47320.25925528207982784No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44300.24270940397583207No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA41320.22638267657520048No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37100.20326227737027924No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA35440.19416752318066566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35200.19285261896048053No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA33300.1824429605506819No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32560.1783886725384445No Hit
GTTTATTACTGTCTGCACCGTAGCAACTGGCCGGGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA28980.15877468458735017No Hit
ATATATTACTGTGCGGGTGGTGGTGACTACTGGTACTTCGATGTCTGGGGCCGTGGCACCCTGGTCACTGTCTC27970.15324112932740458No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27460.15044695785951126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26290.14403679978610892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25740.1410234776148514No Hit
GTCTCCAGCCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACCATTAGCAG25130.1376814293885476No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24780.13576386073411104No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24640.1349968332723364No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23430.12836752449556987No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23020.126121229786087No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACCATTAGCAGCTACTTAGGCTGGTA22970.12584729140688178No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22910.1255185653518355No Hit
CTCCTGCAGGGCCAGTCAGACCATTAGCAGCTACTTAGGCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT22740.12458717486253772No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22600.12382014740076308No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22040.12075203755366454No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21960.12031373614693616No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21910.12003979776773092No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21060.11538284532124207No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20840.11417751645273905No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20700.11341048899096441No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20430.11193122174325619No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20390.11171207103989202No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA20090.11006844076466063No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTGTT19930.10919183795120391No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19420.10639766648331057No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19280.10563063902153595No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19230.1053567006423307No Hit
GTCTGGGACAGATTTCACTCTCACCATCGCCAGCCTAGAGCCTGAAGATT18790.10294604290532469No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18760.10278167987780157No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC18650.10217901544355007No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18580.10179550171266274No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18410.10086411122336497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5200.041.0161481
GGTATCA15600.039.2800141
TGGTATC5750.037.666142
GTATCAA38200.036.3360861
TATCAAC44100.031.2316422
ATCAACG46900.029.8615073
GACAGGT3900.029.5142651
TCAACGC47800.029.2188784
AACGCAG48350.028.9532726
CAACGCA48500.028.7242855
CGCAGAG51550.027.3527628
AGAGTAC52800.026.64043211
GCAGAGT53500.026.2918669
TGTTAGA19000.025.3848770
TCTAGAT2151.7541843E-523.79272570
TCTGGCG30550.023.20305370
GCGTGTT18050.022.78650951
CGTGTTC17800.022.7679852
GTCTTAT902.4171236E-422.5697331
TATAAGA2105.456968E-1222.5604482
Produced by FastQC (version 0.11.5)