FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805902
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC70850.3923247219395073No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCT50600.28019239139222396No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50010.27692532595899444No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA44000.24364555773236865No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40600.22481840099850378No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40050.22177283152684918No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35890.19873725152306163No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCTATGATTACCA34360.19026503099282244No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33980.18816081935786105No Hit
CCCCTGTGCTGGTCATCTATGAGGACACCAAACGACCCTCCGGGATCCCT33620.18616735570368714No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33260.1841738920495132No Hit
CCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG32400.1794117288756533No Hit
CTACTATTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTATTCGGCG31330.17348671190352521No Hit
TCTATGAGGACACCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC30740.17021964647029572No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT30650.16972128055675223No Hit
GTTCAGGGACAATGGCCACCTTGACCATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTATTGTTACTCAA30230.167395572960216No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29430.16296565372871838No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28700.15892335242997682No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27800.15393969329454202No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27580.15272146550588017No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACGG27020.14962052204383183No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26930.14912215613028834No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26870.14878991218792603No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26640.14751631040887048No Hit
CAATAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATGGTCAAGGT25900.14341863511973518No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCGATAGGCAC25890.14336326112934145No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25660.1420896593502859No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25470.14103755353280523No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24910.1379366100707569No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACACCAAACGACCCTCCGG24910.1379366100707569No Hit
GTGTATTACTGTGCGAGAGATTTCCCGACATATTGTGGTGGTGACTGCCA24790.13727212218603224No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24540.13588777242618924No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24340.13478029261831484No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24030.1330636989161095No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23770.1316239751658728No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA23370.129409015550124No Hit
ACATAAAGGAGGATGGAAGTGAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG23130.1280800397806747No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22800.12625269809768191No Hit
GTGCTGGTCATCTATGAGGACACCAAACGACCCTCCGGGATCCCTGAGAG22700.12569895819374471No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA22130.1225426407413027No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22080.12226577078933408No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21670.11999543718319156No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21430.11866646141374228No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21320.11805734751941135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20060.11108022472980261No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG19970.11058185881625912No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC19890.11013886689310937No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG19630.10869914314287266No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19500.10797928126775429No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19280.10676105347909245No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18870.1044907198729499No Hit
GGATGAAGCTGACTACTATTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTATTCGGCGGAGGGACCAAG18840.10432459790176876No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG18770.10393697996901272No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18750.10382623198822527No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18730.10371548400743782No Hit
CCTTTATGTTGGCCACGCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC18650.10327249208428807No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGTGCCTATCGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG18600.10299562213231947No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18590.10294024814192576No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18460.10222038626680739No Hit
CCCTGAGGAGACGGCGACCAGGATTCCCTGGCCCCAGTAGTTGTGGCAGTCACCACCACAATATGTCGGGAAAT18420.10199889030523251No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18250.10105753246853925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3300.057.40004770
GTCGTAT352.2845856E-549.2448139
GTGGTAT3300.046.598951
GGTATCA12900.044.7687031
TGGTATC3600.042.7133372
ACGAGAC5200.040.7419522
CGTATGC4500.040.70080641
CGAGACA5450.040.12813623
TCGCTAA4850.039.55007631
CGCTAAT4600.039.4908532
CACGAGA5200.039.4255121
TCGTATG4650.039.33279840
TCTCGTA3750.038.55515338
GTATCAA35800.038.4679951
TATACAC5550.038.1726383
CGAGCCC5400.037.9621515
ATCGTAT557.4389627E-637.60512539
GTATGCC4800.037.484342
GGATGGG14100.037.42343570
ATGCCGT4850.037.17813544
Produced by FastQC (version 0.11.5)