FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805902
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC70330.3894452744390338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54190.30007165394356944No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46810.259205649033004No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCT46780.25903952706182287No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40960.22681186465267775No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA39250.21734291229535155No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38290.21202700921755446No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35570.19696528383046255No Hit
CCCCTGTGCTGGTCATCTATGAGGACACCAAACGACCCTCCGGGATCCCT32450.17968859882762186No Hit
CTACTATTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTATTCGGCG30910.17116100430698897No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29840.16523598733486092No Hit
CCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG29780.16490374339249858No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29700.16446075146934883No Hit
GTTCAGGGACAATGGCCACCTTGACCATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTATTGTTACTCAA28270.15654227084304684No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCTATGATTACCA28210.15621002690068453No Hit
TCTATGAGGACACCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC27210.1506726278613125No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27040.14973127002461928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26380.14607658665863377No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT26320.1457443427162714No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26050.14424924497564098No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26040.14419387098524727No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACGG26040.14419387098524727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25950.14369550507170378No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25720.1424219032926482No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25650.14203428535989218No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24770.1371613742052448No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACACCAAACGACCCTCCGG24720.1368845042532762No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCGATAGGCAC24400.13511253656067715No Hit
GTGTATTACTGTGCGAGAGATTTCCCGACATATTGTGGTGGTGACTGCCA23410.12963051151169885No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23100.12791391780949354No Hit
GTGCTGGTCATCTATGAGGACACCAAACGACCCTCCGGGATCCCTGAGAG23000.12736017790555634No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22540.1248129743474452No Hit
CAATAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATGGTCAAGGT22300.12348399857799594No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21700.12016155915437272No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21210.11744823362508042No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20860.11551014396130022No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20760.11495640405736303No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20680.11451341213421326No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGTGCCTATCGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG19930.11036036285468424No Hit
ACATAAAGGAGGATGGAAGTGAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG19780.10952975299877844No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC19580.10842227319090404No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19560.10831152521011661No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19530.10814540323893544No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19480.10786853328696684No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19460.10775778530617941No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA19430.10759166333499824No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA19290.10681642746948616No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG19070.10559819968082433No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG18910.1047122158345248No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC18520.1025526302091697No Hit
GGATGAAGCTGACTACTATTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTATTCGGCGGAGGGACCAAG18320.10144515040129531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4750.043.861051
TTTACGG350.001348524139.42556440
GGTATCA14750.039.1324921
GTATCAA38300.038.8803831
TGGTATC5200.038.7399522
GGATGGG16950.035.35101770
TATCAAC43350.033.710492
ATCAACG45950.031.940153
TCAACGC46950.031.2503244
AACGCAG47200.031.2285236
CAACGCA47450.030.9201725
CGCAGAG49850.029.4999838
ACGCAGA49750.029.4906967
AGAGTAC51050.028.87178411
CAGAGTA51700.028.57557510
GCAGAGT51900.028.4662469
GACAGGT3750.028.234051
TCTATAC500.00815232627.305113
GTTGAGT34800.026.44251870
AGGTGAT17400.025.82757670
Produced by FastQC (version 0.11.5)