FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577871
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC57980.36745716221414804No Hit
ATCCTGGGTAGTGCTGGTACCAGTTTACACCATTAGGTCCGATGCTGGAGCTGCTTCCAGAACAAGAGATGGTGA54960.34831744800430453No Hit
ACCCAGGATCGGCCCCCAAACTCCTCATCTATGGCAATAATCAGCGGCCC53060.33627590595175394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52610.3334239617814131No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46010.29159544728307957No Hit
GATTATTACTGTGAAGCATGGGATGACAGCCTGAAAGCCGTGATATTCGG45150.28614506509087245No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42940.2721388503876426No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACGGCTTTCAGG42560.2697305419771325No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC39940.2531258892520365No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGA39390.24964017971050864No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36870.2336692923565995No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35420.22447969447438984No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCGGGCTGTTCATTTGCAGATACA34510.21871242959658932No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34420.21814204076252114No Hit
CCCCAGAGGGCCGCTGATTATTGCCATAGATGAGGAGTTTGGGGGCCGATCCTGGGTAGTGCTGGTACCAGTTTA31140.19735453658759175No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31080.19697427736487966No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30670.19437583934301347No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT30040.19038311750453618No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29160.1848059822380917No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28950.18347507495859927No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28680.18176390845639473No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28430.18017949502842756No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28330.17954572965724067No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28120.17821482237774824No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28110.17815144584062956No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC26690.16915197756977599No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26170.16585639763960425No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26170.16585639763960425No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGACTGAGGTGCCAGACCTCGAGCCAGAGAATCGGTCAGGGACC25060.15882160201942996No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24810.1572371885914628No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGAAGCATGGGATGACAGCCTGAAAGCCGTGATATTCGGCGGAGGGAC24690.15647667014603855No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24490.15520913940366482No Hit
GGGTAGTGCTGGTACCAGTTTACACCATTAGGTCCGATGCTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT24410.1547021271067153No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24300.15400498519840974No Hit
CCATTAGGTCCGATGCTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT23400.1483010968577279No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACGG22660.14361123311094506No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACGGCTTTCAGGC22660.14361123311094506No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22460.14234370236857133No Hit
CCTCTGGGGTCCCTGACCGATTCTCTGGCTCGAGGTCTGGCACCTCAGTCTCCCTGGCCATCAGTGGGCTCCAGT22410.1420268196829779No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22360.14170993699738446No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21390.1355624128968718No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21330.13518215367415967No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21310.1350554005999223No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21120.13385124639466722No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21030.13328085756059907No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21020.13321748102348038No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20980.1329639748750056No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20290.12859099381381622No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19940.12637281501466216No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19650.12453489543822023No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC19600.12421801275262681No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19430.12314061162160912No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19140.12130269204516719No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19040.12066892667398031No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18660.11826061826347022No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18480.11711984059533384No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAAG18270.1157889333158414No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18240.11559880370448535No Hit
CTGATGGCCAGGGAGACTGAGGTGCCAGACCTCGAGCCAGAGAATCGGTC18170.11515516794465455No Hit
GTGTAAACTGGTACCAGCACTACCCAGGATCGGCCCCCAAACTCCTCATC18030.11426789642499291No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18000.11407776681363686No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17990.11401439027651818No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17920.11357075451668736No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17730.1123666003114323No Hit
GCTTGGTCCCTCCGCCGAATATCACGGCTTTCAGGCTGTCATCCCATGCTTCACAGTAATAATCAGCCTCATCCT17310.10970478575244745No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17220.10913439691837926No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC17210.10907102038126057No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17160.10875413769566714No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16840.10672608850786916No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16780.10634582928515703No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16770.10628245274803834No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGTTGGGTCCGCCAGGCTCCGGGGAAGGGG16530.10476141585718986No Hit
GTCATCCCATGCTTCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCC16390.10387414433752822No Hit
GATGAGGAGTTTGGGGGCCGATCCTGGGTAGTGCTGGTACCAGTTTACAC16370.10374739126329086No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA16260.10305024935498529No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGTTGGGTCCG16250.1029868728178666No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16200.10266999013227318No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16190.1026066135951545No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16180.10254323705803581No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16080.10190947168684891No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16000.10140245938989943No Hit
GTCTGGCACCTCAGTCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15890.10070531748159388No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15880.10064194094447519No Hit
GCACTACCCAGGATCGGCCCCCAAACTCCTCATCTATGGCAATAATCAGCGGCCCTCTGGGGTCCCTGACCGATT15800.10013492864752568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGC200.006845454752.7063758
GTGGTAT2200.049.835931
GGTATCA8450.046.2235641
TGGTATC2500.043.855622
GTATCAA23750.040.3933331
TGTTAAC350.001395890339.14935712
TATACAC3200.037.474283
ACGAGAC3250.035.84126722
CGATTAT400.002167921535.79260666
TCGTATG3300.035.55854840
TAGGCAT3500.035.2847429
ATGCCGT3250.035.15366444
TATCAAC27600.034.510482
TTATACA3400.034.26222
CGTATGC3350.034.0247541
TCTCCGA3650.033.7853611
CGAGACT3550.033.77749623
ATCTCCG3700.033.32880410
CTTATAC3500.033.2832831
ATCAACG29000.033.198893
Produced by FastQC (version 0.11.5)