FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577871
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCAGGATCGGCCCCCAAACTCCTCATCTATGGCAATAATCAGCGGCCC58220.3689781991049965No Hit
ATCCTGGGTAGTGCTGGTACCAGTTTACACCATTAGGTCCGATGCTGGAGCTGCTTCCAGAACAAGAGATGGTGA50420.31954450015242053No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50250.3184670990214029No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC46150.2924827188027411No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC43970.27866663371086736No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43890.27815962141391787No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42040.26643496204696077No Hit
GATTATTACTGTGAAGCATGGGATGACAGCCTGAAAGCCGTGATATTCGG41530.26320275865390774No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34870.22099398493286207No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32580.2064807579326827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32410.20540335680166505No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31570.2000797276836953No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACGGCTTTCAGG31150.19741791312471044No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30460.19304493206352102No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30110.190826753264367No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCGGGCTGTTCATTTGCAGATACA29890.18943246944775588No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29220.18518624146080384No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28140.17834157545198562No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27250.17270106364842247No Hit
CCCCAGAGGGCCGCTGATTATTGCCATAGATGAGGAGTTTGGGGGCCGATCCTGGGTAGTGCTGGTACCAGTTTA27170.17219405135147295No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26800.16984911947808154No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26770.16965898986672548No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26430.16750418760469013No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGAAGCATGGGATGACAGCCTGAAAGCCGTGATATTCGGCGGAGGGAC26030.16496912611994263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25970.1645888668972305No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25630.16243406463519516No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC24820.15730056512858148No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24130.15292758406739207No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24020.15223044215908652No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23840.15108966449095015No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23770.15064602873111932No Hit
CCTCTGGGGTCCCTGACCGATTCTCTGGCTCGAGGTCTGGCACCTCAGTCTCCCTGGCCATCAGTGGGCTCCAGT23570.14937849798874558No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23540.14918836837738955No Hit
GGGTAGTGCTGGTACCAGTTTACACCATTAGGTCCGATGCTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT23150.14671668342976074No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT22330.1415198073860284No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22320.1414564308489097No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21120.13385124639466722No Hit
CCATTAGGTCCGATGCTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT21040.13334423409771776No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21010.1331541044863617No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20490.12985852455618996No Hit
GTGTAAACTGGTACCAGCACTACCCAGGATCGGCCCCCAAACTCCTCATC20360.12903462957364703No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC19960.12649956808889953No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19680.12472502504957629No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19240.12193645741635406No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19010.12047879706262425No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18960.12016191437703083No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGA18640.11813386518923284No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACGG18500.11724659366957123No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18470.11705646405821515No Hit
CTCTTGTTCTGGAAGCAGCTCCAGCATCGGACCTAATGGTGTAAACTGGTACCAGCACTACCCAGGATCGGCCCC18060.11445802603634897No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18060.11445802603634897No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18040.1143312729621116No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17820.11293698914550049No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17690.11211309416295755No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17550.11122582264329593No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17410.1103385511236343No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17400.11027517458651562No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAAG17380.11014842151227824No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17110.1084372550100737No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC17030.10793024271312421No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACGGCTTTCAGGC17000.10774011310176815No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGTTGGGTCCGCCAGGCTCCGGGGAAGGGG16830.10666271197075046No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16720.10596557006244489No Hit
CTGATGGCCAGGGAGACTGAGGTGCCAGACCTCGAGCCAGAGAATCGGTC16680.10571206391397016No Hit
GTCTGGCACCTCAGTCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG16650.1055219343026141No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16600.10520505161702065No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGTTGGGTCCG16570.10501492200566459No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC16540.10482479239430854No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16530.10476141585718986No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16510.10463466278295248No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16500.10457128624583378No Hit
GCACTACCCAGGATCGGCCCCCAAACTCCTCATCTATGGCAATAATCAGCGGCCCTCTGGGGTCCCTGACCGATT16420.10406427394888428No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16360.10368401472617217No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16190.1026066135951545No Hit
CTCCTCATCTATGGCAATAATCAGCGGCCCTCTGGGGTCCCTGACCGATT15910.10083207055583124No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15810.10019830518464437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)