FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172391
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33980.2898350464989923No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26610.2269720596626893No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25990.22168372155705732No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24940.21272766508784186No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23680.20198039732478326No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21740.18543301680070898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21650.18466535481763338No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20590.17562400257252062No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19690.16794738274176446No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.15941780515203546No Hit
GAGTTACTCTACCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAACTCAAACGAACTGTGGCTGCACCAT18120.15455594592588992No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16490.1406527344546316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16370.13962918514386413No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16090.13724090341873998No Hit
ACTCTACCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAACTCAAACGA15870.1353643963489996No Hit
GGGTAGAGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAAACTACTGATGCTGAGAGTGAAGT15660.1335731850551565No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15570.1328055230720809No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15000.12794366384593536No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14030.1196699735838982No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13820.11787876229005512No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13580.11583166366852014No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13480.11497870590954724No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13260.11310219883980685No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13200.11259042418442311No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT13160.11224924108083395No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13160.11224924108083395No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13010.1109698044423746No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12870.10977566357981254No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12860.10969036780391524No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGACCTCAGATTCAGGGAAA12770.10892270582083963No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12550.10704619875109925No Hit
GATGTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA12370.10551087478494801No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11780.10047842400700789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA1501.8189894E-1145.5506370
CGTATGC4100.042.09443741
TATACAC5150.041.9732133
TTATACA5250.041.817062
TCGTATG4000.041.3272440
CCGTCTT4150.040.4135847
CTTATAC5700.039.700811
AATCGCG350.001400112839.12442836
CCGCTAG700.001413869339.04339670
GTATGCC4450.038.07269742
ATGCCGT4650.038.06026544
GTATCAA46200.037.5037651
CGAGACA5950.036.92891323
CCCACGA5900.036.6485819
ATCTCGT3650.036.62933737
CGTATTG501.3845456E-436.28103661
CTCGTAT3700.036.24262239
GTGGTAT5150.036.070731
ATCTCCG6100.035.98241810
AGCTACG400.002263667335.4779353
Produced by FastQC (version 0.11.5)