FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172391
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41690.355598089715803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26130.2228778624196194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26020.2219396088847492No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24930.21264236931194455No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22550.19234197464838948No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20980.1789505378325149No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18460.15745600230639778No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18460.15745600230639778No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18360.15660304454742488No Hit
GAGTTACTCTACCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAACTCAAACGAACTGTGGCTGCACCAT18100.15438535437409534No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17460.14892642471666875No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17420.1485852416130796No Hit
GGGTAGAGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAAACTACTGATGCTGAGAGTGAAGT15500.13220845264079986No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15170.12939369203618928No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15080.12862603005311368No Hit
GGGTGGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14540.12402005815466001No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14460.12333769194748168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14120.1204376355669738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14040.11975526935979548No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14040.11975526935979548No Hit
ACTCTACCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAACTCAAACGA13410.11438163547826621No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13170.11233453685673125No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13000.1108845086664773No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12930.11028743823519628No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12500.1066197198716128No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12370.10551087478494801No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12140.10354907193931034No Hit
GATGTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA11790.1005637197829052No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGACCTCAGATTCAGGGAAA11770.10039312823111061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47100.039.318611
GTGGTAT6300.038.6216161
ATCAACG56850.032.6690863
TATCAAC57400.032.3123972
TCAACGC57850.032.153134
AACGCAG57950.032.1517876
CAACGCA58450.031.876755
ACGTGTC3900.031.78097263
CGTGTCA3700.031.6449764
GACGTGT3850.031.1406762
CGCAGAG60850.030.564028
TACCGAA450.004717327730.53988338
ACGCAGA61450.030.375457
GGGTACG450.005122157330.03005618
AGAGTAC62000.029.77553211
GCAGAGT63150.029.4508429
GGACGTG4150.028.8180961
CAGAGTA65350.028.19992810
GGTATCA26950.027.837661
GCGATTT1050.0095003626.45850470
Produced by FastQC (version 0.11.5)