FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779967
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT51020.28663452749404905No Hit
GTACCATACTGCTGACAGTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTCTT44390.2493866459321999No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGA43410.2438809258823338No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43100.24213932056043733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41930.23556616499069927No Hit
CAGTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTCTTCCAGACCCACTGCCA34760.1952845193197402No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32590.18309328206646527No Hit
GAGTATTACCAGCAGTTACTTAGCCTGGTACCACCAGAAACCTGGCCAGG32180.18078986857621518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31840.17887972080381265No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA30820.17314927748660508No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29590.1662390370158548No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28870.16219401820370827No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27250.15309272587637862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27240.15303654505954323No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26670.14983423849992725No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25430.1428678172123416No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG25090.14095766943993904No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25050.14073294617259757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24840.13955314901905486No Hit
GTACTAGGGTACTTTAACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC24010.1348901412217193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23030.12938442117185317No Hit
GTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTCTTCCAGACCCACTGCCA22790.12803608156780436No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22500.12640683787957868No Hit
CTCCTGCAGGGCCAGTCAGAGTATTACCAGCAGTTACTTAGCCTGGTACC22400.125845029711225No Hit
GTCTCTGGTGACTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC22150.12444050929034077No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22110.12421578602299929No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21420.12033930966135889No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21200.11910333169098078No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT20780.11674373738389532No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20410.1146650471609867No Hit
CAATAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTA20310.11410323899263301No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20170.11331670755693785No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19990.11230545285390123No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19900.11179982550238292No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19700.11067620916567554No Hit
CCCTAGTACCAACCACTGCTGACGATGTCTCGCACAATAATACACGGCCG19440.10921550792795598No Hit
GTATTATTGTGCGAGACATCGTCAGCAGTGGTTGGTACTAGGGTACTTTAACTACTGGGGCCAGGGAACCCTGGT19260.10820425322491933No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTACCAG19120.10741772178922418No Hit
GGGTATATCTATGACAGTGGGAGCACCAGGTACAACCCCTCCCTCAAGAG18860.1059570205515046No Hit
CATCTATGGTGCATATAGTAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAAGAGACTTCA18850.10590083973466924No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18790.10556375483365703No Hit
GTACCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA18530.10410305359593747No Hit
GTGAGGTACCATACTGCTGACAGTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT18510.10399069196226672No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC18210.10230526745720567No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18090.10163109765518123No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17800.10000185396695557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3750.042.25596240
GGTATCA21550.041.245631
ATGCCGT3500.040.6934844
CGTATGC3800.039.0620841
GTATCAA52000.038.4108241
GCCGTCT3650.037.26189846
ATCTCGT3800.036.96829637
ACGAGAC5050.036.14575222
CTTATAC5100.035.7843441
CCGTCTT3950.035.38348847
AATCTCG4150.033.81002836
GTATGCC4400.033.79751642
GGCAATC4250.033.67790233
CACGAGA5600.032.59114521
TATCAAC61350.032.5559352
AGGCAAT5350.032.44223432
TCAACGC62750.031.8286934
ATCAACG63000.031.7023893
CAACGCA63250.031.6296275
AACGCAG64100.031.1574826
Produced by FastQC (version 0.11.5)