FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779967
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT45920.2579823109080112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45920.2579823109080112No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41120.23101551882703442No Hit
GTACCATACTGCTGACAGTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTCTT40920.2298919024903271No Hit
GTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGA38190.21455453949427153No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35020.1967452205574598No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32590.18309328206646527No Hit
CAGTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTCTTCCAGACCCACTGCCA31690.17803700855128213No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30790.17298073503609898No Hit
GAGTATTACCAGCAGTTACTTAGCCTGGTACCACCAGAAACCTGGCCAGG30530.1715200337983794No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30520.17146385298154404No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA28160.1582051802083971No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24760.1391037024843719No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23990.13477777958804854No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23630.13275527018197528No Hit
GTAATACACTGCCAGATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTCTTCCAGACCCACTGCCA23290.13084512240957277No Hit
GTACTAGGGTACTTTAACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC23000.1292158787213471No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22640.1271933693152738No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21940.12326071213679805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21730.12208091498325531No Hit
CTCCTGCAGGGCCAGTCAGAGTATTACCAGCAGTTACTTAGCCTGGTACC21350.11994604394351131No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20690.116238110032377No Hit
GTCTCTGGTGACTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC20650.11601338676503553No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20450.11488977042832817No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20170.11331670755693785No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20120.113035803472761No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19980.11224927203706586No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19600.11011440099732185No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19050.10702445607137662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19040.10696827525454125No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18860.1059570205515046No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18790.10556375483365703No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18590.10444013849694965No Hit
CCCTAGTACCAACCACTGCTGACGATGTCTCGCACAATAATACACGGCCG18530.10410305359593747No Hit
CAATAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTA18210.10230526745720567No Hit
GGGTATATCTATGACAGTGGGAGCACCAGGTACAACCCCTCCCTCAAGAG18100.10168727847201661No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17940.10078838540265073No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTACCAG17890.10050748131847388No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17810.10005803478379094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.041.2495351
GTATCAA53750.035.9648861
TATCAAC64100.029.9359232
CGGGATA3950.029.92701517
ATCAACG64800.029.7555563
TCAACGC64800.029.6413364
CAACGCA65700.029.1822745
AACGCAG65950.029.071656
ACGCAGA69700.027.4106777
CGCAGAG69850.027.3518188
TCGGTTT1357.581972E-427.14671170
GCTTGTA2750.027.036541
CTAGACA757.15084E-527.0053944
AGAGTAC71750.026.6260211
GCAGAGT73550.025.9751289
CAGTTCG19450.024.11800470
TGTTAGA16500.022.65516570
GGGATAC4600.022.03136318
ACGGGAT7550.021.91758516
AGTACGG68900.021.60931213
Produced by FastQC (version 0.11.5)