FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096877
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29470.26867187478632515No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25530.2327517123615501No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23920.21807367644685777No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC22670.2066776858298606No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21430.19537286313779942No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19690.17950964419893936No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19500.1777774536251558No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19020.17340139322822887No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18830.1716692026544453No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18680.17030168378040564No Hit
ATCTGGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18630.16984584415572573No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18540.16902533283130197No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18330.16711080640764645No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17900.1631905856353994No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG17810.1623700743109756No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17530.15981737241276825No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCCAGATTGTTG17100.15589715164052123No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17050.15544131201584133No Hit
GATTATTACTGCAACTCATATGCAGGCAGCAACAATCTGGGGGTGTTCGG16190.1476008704713473No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15260.13912225345230142No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15240.13893991760242944No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15100.13766356665332577No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGACGTT15090.13757239872838978No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15040.1371165591037099No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15010.13684305532890195No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14820.13511086475511838No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAACTCATAT14750.13447268928056658No Hit
GTCTCTGGTGGCTCCATGAGTATTAATAGTTTCTGTTGGGGCTGGATCCG14720.13419918550575863No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14630.1333786741813348No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14430.13155531568261528No Hit
ATAATACACAGCTGTGTCTCCGGCACTCACATAGCTCAGTCTCAAGGAGAACTGGTTCTTGGACGTGTCTACGGA14400.13128181190780736No Hit
GTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA14140.12891144585947192No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13890.1266322477360725No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13830.12608524018645664No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13490.1229855307386334No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13210.12043282884042605No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13190.12025049299055408No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13050.1189741420414504No Hit
CCCCCAGATTGTTGCTGCCTGCATATGAGTTGCAGTAATAATCAGCCTCA12920.1177889590172827No Hit
CTCCTGCACTGGAAGCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC12710.11587443259362719No Hit
CAATAATACACAGCTGTGTCTCCGGCACTCACATAGCTCAGTCTCAAGGA12660.1154185929689473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12350.11259238729593199No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12090.11022202124759657No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA11980.10921917407330083No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11850.10803399104913312No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11800.10757815142445325No Hit
GGATGAGGCTGATTATTACTGCAACTCATATGCAGGCAGCAACAATCTGGGGGTGTTCGGCGGAGGGACCAAG11730.1069399759499014No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11610.10584596085066968No Hit
GTATTAATAGTTTCTGTTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGG11480.10466077782650196No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11410.10402260235195013No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAG11290.1029285872527184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)